Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Flow cytometry [1]
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- Product number
- M00421 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Mouse IL-17A Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal 20B4.G10.F5 IgG2a kappa antibody for IL 17/IL17A detection. Host: Rat.Size: 100μL. Tested applications: Flow Cytometry. Reactive species: Mouse. IL 17/IL17A information: Molecular Weight: 17504 MW; Subcellular Localization: Secreted; Tissue Specificity: Restricted to activated memory T-cells.
- Reactivity
- Mouse
- Host
- Rat
- Antibody clone number
- 20B4.G10.F5 IgG2a kappa
- Vial size
- 100μL
- Concentration
- 2.94 mg/mL by UV absorbance at 280 nm
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Supportive validation
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- Boster Biological Technology (provider)
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- Experimental details
- Monoclonal anti IL-17A was used to detect IL17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 μg/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 μg/mL anti-mouse IFN over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 μg/mL, washed, fixed and permeabilized and incubated with rat anti mouse IL-17A monoclonal Antibody (Catalog # M00421) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry.
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