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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC7211P-100UG - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-17/IL-17A PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-17/IL-17A in direct ELISAs.
- Reactivity
- Mouse
- Host
- Rat
- Antigen sequence
Q62386- Isotype
- IgG
- Antibody clone number
- 881309
- Vial size
- 100 ug
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of IL-17/IL-17A in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) and either (A) Rat Anti-Mouse IL-17/IL-17A PE-conjugated Monoclonal Antibody (Catalog # IC7211P) or (B) Rat IgG2B Phycoerythrin Isotype Control (Catalog # IC013P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
