Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB3171R-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-17/IL-17A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human IL-17 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer and no cross-reactivity with rhIL-17F, recombinant mouse IL-17, and recombinant rat IL-17A is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q16552
- Isotype
- IgG
- Antibody clone number
- 41802R
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-17/IL-17A by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-17/IL-17A. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-17/IL-17A Monoclonal Antibody (Catalog # MAB3171R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for IL-17/IL-17A at approximately 14-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-17/IL-17A by Western Blot. Western blot shows lysates of human CD4+ T cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-17/IL-17A Monoclonal Antibody (Catalog # MAB3171R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-17/IL-17A at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-17/IL-17A in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL calcium ionomycin for 16 hours, then stained with Mouse Anti-Human IL-17/IL-17A Monoclonal Antibody (Catalog # MAB3171R, dark orange filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.