A00421-2

antibody from Boster Biological Technology

Targeting: IL17A CTLA8, IL-17, IL-17A, IL17

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A00421-2

Provider

Boster Biological Technology

Product name

Anti-IL17/Il17a Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for IL 17/Il17a detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. IL 17/Il17a information: Subcellular Localization: Secreted; Tissue Specificity: Restricted to a subset of activated T-cells.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Mechanic study based on untargeted metabolomics of Pi-pa-run-fei-tang on pepper combined with ammonia induced chronic cough model mice.

Jie XL, Tong ZR, Xu XY, Wu JH, Jiang XL, Tao Y, Feng PS, Yu J, Lan JP, Wang P
Journal of ethnopharmacology 2024 May 23;326:117905

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Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration.

Tan J, Zhuo Z, Wang X, Zhang Y, Qian Y, Lin F
International journal of molecular medicine 2023 Nov;52(5)

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IL-23/IL-17 immune axis mediates the imiquimod-induced psoriatic inflammation by activating ACT1/TRAF6/TAK1/NF-κB pathway in macrophages and keratinocytes.

Chen WC, Wen CH, Wang M, Xiao ZD, Zhang ZZ, Wu CL, Wu R
The Kaohsiung journal of medical sciences 2023 Aug;39(8):789-800

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A Synbiotic Ameliorates Con A-Induced Autoimmune Hepatitis in Mice through Modulation of Gut Microbiota and Immune Imbalance.

Liu Q, Yang H, Kang X, Tian H, Kang Y, Li L, Yang X, Liu H, Ren P, Kuang X, Tong M, Fan W
Molecular nutrition & food research 2023 Apr;67(7):e2200428

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IL-17-induced HIF1α drives resistance to anti-PD-L1 via fibroblast-mediated immune exclusion.

Chen X, Zhao J, Herjan T, Hong L, Liao Y, Liu C, Vasu K, Wang H, Thompson A, Fox PL, Gastman BR, Li X, Li X
The Journal of experimental medicine 2022 Jun 6;219(6)

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Oral exposure to dibutyl phthalate exacerbates chronic lymphocytic thyroiditis through oxidative stress in female Wistar rats.

Wu Y, Li J, Yan B, Zhu Y, Liu X, Chen M, Li D, Lee CC, Yang X, Ma P
Scientific reports 2017 Nov 13;7(1):15469

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Inhibition of Tumor Growth and Immunomodulatory Effects of Flavonoids and Scutebarbatines of Scutellaria barbata D. Don in Lewis-Bearing C57BL/6 Mice.

Gong T, Wang CF, Yuan JR, Li Y, Gu JF, Zhao BJ, Zhang L, Jia XB, Feng L, Liu SL
Evidence-based complementary and alternative medicine : eCAM 2015;2015:630760

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Th17 cells are associated with the Th1/Th2‑cell balance during Echinococcus multilocularis infection.

Ma X, Wang L, Zhao H, Pang N, Zhang F, Jiang T, Liu X, Mamuti W, Wen H, Ding J
Molecular medicine reports 2014 Jul;10(1):236-40

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of IL17 using anti-IL17 antibody (A00421-2). IL17 was detected in paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IL17 Antibody (A00421-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image