IC317N

antibody from R&D Systems

Targeting: IL17A CTLA8, IL-17, IL-17A, IL17

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC317N

Provider

R&D Systems

Product name

Human IL-17/IL-17A Alexa Fluor® 700-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human IL-17 in direct ELISAs. In direct ELISAs, approximately 12% cross-reactivity with recombinant canine IL-17 is observed and 25%-50% reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.

Reactivity

Human

Host

Mouse

Conjugate

Near infrared dye

Antigen sequence

Q16552


Isotype

IgG

Antibody clone number

41809

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of IL-17/IL-17A in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Mouse Anti-Human IL-17/IL-17A Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # IC317N) and Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # IC0041N). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.