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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP2-59328 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Kv3.2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects 80-100kDa. Varies due to post-translational modifications.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Store at -20C.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Kv3.2 Antibody (S410-17) [NBP2-59328] - Analysis of Mouse, Rat Brain showing detection of ~80-100 kDa Kv3.2 protein using Mouse Anti-Kv3.2 Monoclonal Antibody, Clone S410-17. Lane 1: MW Ladder. Lane 2: Mouse Brain. Lane 3: Rat Brain Membrane.Lane 4: Rat Brain. Load: 20 ug . Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Kv3.2 Monoclonal Antibody at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~80-100 kDa. Other Band(s): ~30 kDa for Rat Brain .
