Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- HPA006731 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA006731, RRID:AB_1080495
- Product name
- Anti-USP10
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human USP10, Gene description: ubiquitin specific peptidase 10, Alternative Gene Names: KIAA0190, UBPO, Validated applications: WB, IHC, ICC, Uniprot ID: Q14694, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.4 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references The optineurin/TIA1 pathway inhibits aberrant stress granule formation and reduces ubiquitinated TDP-43
Processing of the ribosomal ubiquitin-like fusion protein FUBI-eS30/FAU is required for 40S maturation and depends on USP36
USP10 is a critical factor for Tau-positive stress granule formation in neuronal cells
G3BP1 inhibits ubiquitinated protein aggregations induced by p62 and USP10
Kakihana T, Takahashi M, Katsuragi Y, Yamashita S, Sango J, Kanki T, Onodera O, Fujii M
iScience 2021;24(7):102733
iScience 2021;24(7):102733
Processing of the ribosomal ubiquitin-like fusion protein FUBI-eS30/FAU is required for 40S maturation and depends on USP36
van den Heuvel J, Ashiono C, Gillet L, Dörner K, Wyler E, Zemp I, Kutay U
eLife 2021;10
eLife 2021;10
USP10 is a critical factor for Tau-positive stress granule formation in neuronal cells
Piatnitskaia S, Takahashi M, Kitaura H, Katsuragi Y, Kakihana T, Zhang L, Kakita A, Iwakura Y, Nawa H, Miura T, Ikeuchi T, Hara T, Fujii M
Scientific Reports 2019;9(1)
Scientific Reports 2019;9(1)
G3BP1 inhibits ubiquitinated protein aggregations induced by p62 and USP10
Anisimov S, Takahashi M, Kakihana T, Katsuragi Y, Kitaura H, Zhang L, Kakita A, Fujii M
Scientific Reports 2019;9(1)
Scientific Reports 2019;9(1)
No comments: Submit comment
Enhanced validation
Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting USP10 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting USP10 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:461
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-USP10 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
Enhanced validation
- Submitted by
- 55af80e3e0991
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Confocal images of immunofluorescently stained human U-2 OS cells.The protein USP10 is shown in green. The image to the left show cells transfected with control siRNA and the image to the right show cells where USP10 has been downregulated with specific siRNA.
- Sample type
- U-2 OS cells
- Primary Ab dilution
- 1:200
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:800
- Knockdown/Genetic Approaches Application
- Immunocytochemistry
