14-9806-82

antibody from Invitrogen Antibodies

Targeting: HNRNPUL1 E1B-AP5, E1BAP5, FLJ12944, HNRPUL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

14-9806-82

Provider

Invitrogen Antibodies

Product name

hnRNP UL1 Monoclonal Antibody (EAP 4A11), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: This EAP 4A11 monoclonal antibody recognizes human hnRNP UL1. EAP 4A11 detects a ~95 kDa band corresponding to hnRNP UL1 by Western Blot and stains hnRNP UL1 in the nucleus in Immunocytochemistry. Applications Reported: This EAP 4A11 antibody has been reported for use in western blot and immunocytochemistry/immunofluorescence. Applications Tested: This EAP 4A11 antibody has been tested by western blot of human cell lysates and immunocytochemistry/immunofluorescence of human cell lines. This may be used at less than or equal to 4.0 µg/ml for western blot and less than or equal to 2.5 µg/mL for immunocytochemistry/immunofluorescence.

Reactivity

Human

Host

Rat

Isotype

IgG

Antibody clone number

EAP 4A11

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis was performed on whole cell extracts using 10 µg lysate of HEL (Lane 1), Daudi (Lane 2), 293T (Lane 3), HeLa (Lane 4), and NIH 3T3 (Lane 5). The blot was probed with Anti-hnRNP UL1 Monoclonal antibody clone EAP 4A11 (2 µg/ml) and detected by chemiluminescence using Goat-anti Rat IgG (H+L) Secondary Antibody, HRP (Product # 31471) at a 1: 4,000 dilution. Visualization using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34578). The blot was reprobed with beta Tubulin Loading Control Antibody (Product # MA5-16308) and Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP (Product # 31430)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of hnRNP UL1 Monoclonal Antibody (Product # 14-9806-82) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 30 minutes at 37°C, and blocked with 10% Normal Goat Serum for 1 hour at room temperature. The cells were labeled with 1.25 µg/mL of hnRNP UL1 Monoclonal Antibody (Product # 14-9806-82) in 0.1% Triton X-100, incubated overnight at 4°C and then labeled with 4 µg/mL of Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 conjugate (Product # A11006) for 1 hour at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with Fluoromount G with DAPI (Product # 00-4959). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents a merged image of control cells incubated with 1.25 µg/mL of Rat IgG2b Isotype Control (Product # 14-4031-85) antibody to assess background. The images were captured at 40X magnification.