MAB5044

antibody from Novus Biologicals

Targeting: FOXC2 FKHL14, MFH-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

MAB5044

Provider

Novus Biologicals

Product name

Mouse Monoclonal FoxC2 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human FoxC2 in direct ELISAs and Western blots. Approximately 25% cross-reactivity with recombinant human (rh) FoxA1 (aa 253-472) is observed in Western blots but not in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with rhFoxB1 (aa 191-324), D3 (aa 1-140), F1 (aa 129-354), J1 (aa 306-421), K1 (aa 493-670), M1 (aa 642-763), P1 (aa 231-420), P2 (aa 269-460), P3 (aa 179-279), or P4 (aa 229-410) is observed.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Hemodynamic regulation of perivalvular endothelial gene expression prevents deep venous thrombosis.

Welsh JD, Hoofnagle MH, Bamezai S, Oxendine M, Lim L, Hall JD, Yang J, Schultz S, Engel JD, Kume T, Oliver G, Jimenez JM, Kahn ML
The Journal of clinical investigation 2019 Dec 2;129(12):5489-5500

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human FoxC2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, T47D human breast cancer cell line, and MDA-MB-453 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human FoxC2 Monoclonal Antibody (Catalog # MAB5044) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FoxC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.