PA5-36261

antibody from Invitrogen Antibodies

Targeting: FOXC2 FKHL14, MFH-1

Antibody data

Submit

Validation data

Product number: PA5-36261 - Provider product page
Provider: Invitrogen Antibodies
Product name: FOXC1/FOXC2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody detects endogenous protein at a molecular weight of 57 kDa.
Concentration: 1 mg/mL

FOXC2 protein structure - PA5-36261 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of FOXC1/FOXC2 in Lane 1: 3T3-L1 whole cell lysate (40 µg), Lane 2: C6 whole cell lysate (40 µg), Lane 3: A549 whole cell lysate (40 µg), Lane 4: HeLa whole cell lysate (40 µg), Lane 5: HEK293T whole cell lysate (40 µg). Samples were incubated with FOXC1/FOXC2 polyclonal antibody (Product # PA5-36261) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of FOXC1+FOXC2 using FOXC1+FOXC2 polyclonal antibody (Product # PA5-36261) at a dilution of 1:500. Lane 1: Hela whole cell lysate, Lane 2: HEK293T whole cell lysate, Lane 3: PC12 whole cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-FOXC1/FOXC2 Polyclonal Antibody (Product # PA5-36261) and a 70 kDa band corresponding to Forkhead box protein C1 was observed across the tested cell lines, and expression was higher upon treating A549 with CoCl2. Nuclear enriched extracts (40 µg lysate) of A549 (Lane 1), A549 (CoCl2, 50uM, 24 hrs)(Lane 2), HeLa (Lane 3), MCF 10A (Lane 4), MIA PaCa-2 (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1/4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).