AP16142PU-T

antibody from Acris Antibodies GmbH

Targeting: FOXC2 FKHL14, MFH-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP16142PU-T

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP16142PU-T, RRID:AB_10976070

Product name

anti FOXC2 / FKHL14 / MFH1 (C-term)

Antibody type

Polyclonal

Antigen

Peptide from C-Terminus of the protein sequence according to NP_005242.1

Reactivity

Human, Mouse, Rat, Bovine, Canine

Host

Goat

Vial size

20 µg

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP16142PU-N staining (1µg/ml) of Human Breast Cancer lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

HEK293 lysate (10ug protein in RIPA buffer) over expressing Human FOXC2 with DYKDDDDK tag probed with AP16142PU-N(0.1 µg/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AP16142PU-N (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Staining of Paraffin Embedded Human Breast cancer using AP16142PU-N at 2 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.