AF5044

antibody from R&D Systems

Targeting: FOXC2 FKHL14, MFH-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5044

Provider

R&D Systems

Product name

Human FoxC2 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human FoxC2 in Western blots.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

Q99958


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Piezo1 incorporates mechanical force signals into the genetic program that governs lymphatic valve development and maintenance.

Choi D, Park E, Jung E, Cha B, Lee S, Yu J, Kim PM, Lee S, Hong YJ, Koh CJ, Cho CW, Wu Y, Li Jeon N, Wong AK, Shin L, Kumar SR, Bermejo-Moreno I, Srinivasan RS, Cho IT, Hong YK
JCI insight 2019 Mar 7;4(5)

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Epithelial-mesenchymal transitioned circulating tumor cells capture for detecting tumor progression.

Satelli A, Mitra A, Brownlee Z, Xia X, Bellister S, Overman MJ, Kopetz S, Ellis LM, Meng QH, Li S
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Feb 15;21(4):899-906

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human FoxC2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human FoxC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5044) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for FoxC2 was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human FoxC2 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for FoxC2 at approximately 73 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human FoxC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5044) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

FoxC2 in HeLa Human Cell Line. FoxC2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human FoxC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5044) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.