2299-MSM2-P1

antibody from Invitrogen Antibodies

Targeting: FOXL1 FKH6, FKHL11, FREAC7

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Gel shift (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

2299-MSM2-P1

Provider

Invitrogen Antibodies

Product name

FOXL1/Forkhead Box Protein L1 (Transcription Factor) Monoclonal Antibody (PCRP-FOXL1-1F8)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

PCRP-FOXL1-1F8

Vial size

100 µg

Concentration

200 µg/mL

Storage

4° C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PFA-fixed HeLa cells. FOXL1 Mouse Monoclonal Antibody (PCRP-FOXL1-1F8) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human prostate stained. FOXL1 Mouse Monoclonal Antibody (PCRP-FOXL1-1F8) at 2 µg/mL. HIER: Tris/EDTA, pH9.0, 45 min. 2nd Ab: HRP-polymer, 30 min. DAB, 5 min.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of PFA-fixed HeLa cells. FOXL1 Mouse Monoclonal Antibody (PCRP-FOXL1-1F8) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Analysis of Protein Array containing more than 19,000 full-length human proteins using FOXL1 Mouse Monoclonal Antibody (PCRP-FOXL1-1F8). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SDS-PAGE Analysis Purified FOXL1 Mouse Monoclonal Antibody (PCRP-FOXL1-1F8). Confirmation of Purity and Integrity of Antibody.