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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- PA5-47701 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KAZALD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse IGFBP-rP10 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-human IGFBP-rP10 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47701) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for IGFBP-rP10 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KAZALD1 in LNCaP human prostate cancer cell line. Samples were incubated in KAZALD1 polyclonal antibody (Product # PA5-47701) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti‚Sheep IgG secondary antibody. A specific band was detected for IGFBP-rP10 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KAZALD1 in LNCaP human prostate cancer cell line. Samples were incubated in KAZALD1 polyclonal antibody (Product # PA5-47701) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti‚Sheep IgG secondary antibody. A specific band was detected for IGFBP-rP10 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions.
