PA5-57370

antibody from Invitrogen Antibodies

Targeting: HMGB4 FLJ40388

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-57370

Provider

Invitrogen Antibodies

Product name

HMGB4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PPSSFLLFCQ DHYAQLKREN PNWSVVQVAK ATGKMWSTAT DLEKHPYEQR VALLRAKYFE ELELYRKQCN ARKKYRMS

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HMGB4 in control (vector only transfected HEK293T lysate) and HMGB4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a HMGB4 Polyclonal Antibody (Product # PA5-57370).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HMGB4 in human testis tissue shows strong nuclear positivity in a subset of cells in seminiferus ducts. Samples were probed using a HMGB4 Polyclonal Antibody (Product # PA5-57370).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HMGB4 in human testis and endometrium tissues using HMGB4 Polyclonal Antibody (Product # PA5-57370). Corresponding HMGB4 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HMGB4 in human endometrium using HMGB4 Polyclonal Antibody (Product # PA5-57370) shows low expression as expected.