Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP1-93780 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-93780, RRID:AB_11007045
- Product name
- Rabbit Polyclonal HMGB4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human HMGB4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics.
Fagerberg L, Hallström BM, Oksvold P, Kampf C, Djureinovic D, Odeberg J, Habuka M, Tahmasebpoor S, Danielsson A, Edlund K, Asplund A, Sjöstedt E, Lundberg E, Szigyarto CA, Skogs M, Takanen JO, Berling H, Tegel H, Mulder J, Nilsson P, Schwenk JM, Lindskog C, Danielsson F, Mardinoglu A, Sivertsson A, von Feilitzen K, Forsberg M, Zwahlen M, Olsson I, Navani S, Huss M, Nielsen J, Ponten F, Uhlén M
Molecular & cellular proteomics : MCP 2014 Feb;13(2):397-406
Molecular & cellular proteomics : MCP 2014 Feb;13(2):397-406
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HMGB4 Antibody [NBP1-93780] - Analysis in control (vector only transfected HEK293T lysate) and HMGB4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HMGB4 Antibody [NBP1-93780] - Staining of human testis shows strong nuclear positivity in a subset of cells in seminiferus ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HMGB4 Antibody [NBP1-93780] - Staining of human endometrium shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HMGB4 Antibody [NBP1-93780] - Staining in human testis and endometrium tissues using anti-HMGB4 antibody. Corresponding HMGB4 RNA-seq data are presented for the same tissues.