10473-1-AP

antibody from Proteintech Group

Targeting: USP5 IsoT

Provider product page for 10473-1-AP

Western blot

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Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [65]
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Validations
Western blot [1]

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Product number: 10473-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10473-1-AP, RRID:AB_2272754
Product name: USP5 antibody
Antibody type: Polyclonal
Description: KD/KO validated USP5 antibody (Cat. #10473-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

USP5 protein structure - 10473-1-AP shown in red.

Submitted references USP5-mediated CD73 deubiquitination drives osimertinib resistance via PI3K/AKT and glycolysis activation in LUAD.

Chen R, Han XH, Zhang Z, Han XJ, Xie J
iScience 2026 Mar 20;29(3):114916

USP5 inhibition stimulates immunogenic ferroptosis that enhances immunotherapy in diffuse large B-cell lymphoma.

Wu J, Liu C, Chen Q, Deng Y, Wang J, Gao Y, Wei B
Cell communication and signaling : CCS 2026 Jan 5;24(1):93

MGMT upregulation mediates Temozolomide resistance conferred USP5 dependency.

Bhardwaj S, Sanjay, Sharma D, Taneja L, Yadav BK, Yadav AK
Scientific reports 2026 Jan 24;16(1):6118

α-hederin Targets USP5 to Inhibit Colorectal Tumorigenesis by Disrupting STAT3 Deubiquitination.

Feng H, Wang Q, Li L, Tao L, Zeng S, Li Z, Fan M, Yu C, Sun D, Shen W, Cheng H
International journal of biological sciences 2025;21(15):6697-6722

TFEB promotes Ginkgetin-induced ferroptosis via TRIM25 mediated GPX4 lysosomal degradation in EGFR wide-type lung adenocarcinoma.

Wang HJ, Dong LF, Ding LL, Miao XY, Zhang YW, Zhao LP, Yu LH, Guan ZR, Jiang YP, Tang XQ, Yan YX, Lou JS
Theranostics 2025;15(7):2991-3012

USP5-mediated stabilization of ILF2 via deubiquitination drives the tumor growth of colorectal cancer.

You W, Xu X, Ye J, Cui X, Han K, Chen G, Yang P, Yang Y
American journal of cancer research 2025;15(12):5281-5292

Phlorotannins from edible marine seaweed Ecklonia stolonifera disrupt USP5-Cav3.2 calcium channel interactions and reverse chronic inflammatory pain.

Ali MY, Gadotti VM, Antunes FTT, Garcia-Caballero A, Jung HA, Choi JS, Zamponi GW
Toxicology and applied pharmacology 2025 Sep;502:117440

Inhibition of USP5 Attenuates Atherosclerosis by Suppressing PDCD4-Mediated Endothelial Dysfunction: Evidence from In Vitro HUVEC and In Vivo Models.

Chen Y, Wang Y, Wu T, Guo X, Bai X, Wang L, Yan P, Zheng Z, Xiao L, Li L
Cardiovascular toxicology 2025 Sep;25(9):1272-1287

FASN inhibits ferroptosis in breast cancer via USP5 palmitoylation-dependent regulation of GPX4 deubiquitination.

Qian Z, Jiang Y, Cai Y, Gao E, Wang C, Dong J, Wang F, Liu L, Wu D, Zhang F, Wang Y, Ning X, Li Q, You Y, Gu Y, Mei J, Zhao X, Zhang Y
Journal of experimental & clinical cancer research : CR 2025 Oct 14;44(1):289

TRAIL-resistant glioma cells resist Withania somnifera mediated apoptosis via USP5 upregulation.

Bhardwaj S, Yadav AK
Scientific reports 2025 Oct 1;15(1):34133

USP5 regulates ferroptosis in colorectal cancer by targeting the YBX3/SLC7A11 axis through lysosomal degradation.

Qiu H, Liu Y, Zhou H, Hu L, Qi W, Ma H, Liu Y, Li L, Yang N, Huang M, Du R, Meng L, Shi F, Wang B, Yu L, Zhang X, Li G
Cell death & disease 2025 Nov 10;16(1):822

ANXA1 promotes intrahepatic cholangiocarcinoma proliferation and growth by regulating glutamine metabolism through GOT1 stabilization.

Gong Y, Chen L, Wang H, Zheng D, Li F, Wu C, Li Y, Deng Y, He Z, Yu C
Journal of experimental & clinical cancer research : CR 2025 May 19;44(1):151

SP1 promotes triple-negative breast cancer progression by targeting USP5.

Wu SY, Peng ZM, Deng FY, Xiong JY, Luo PY, Han XJ, Zhang Z
Cancer cell international 2025 May 15;25(1):177

USP5 Suppresses Ferroptosis in Bladder Cancer Through Stabilization of GPX4.

Liu C, Deng Y, Huang L, Nie X, Jiang Y, Zhang X, Zhang H
Current issues in molecular biology 2025 Mar 20;47(3)

The deubiquitinase USP24 suppresses ferroptosis in triple-negative breast cancer by stabilizing DHODH protein.

Yang L, An X, Yang S, Lin X, Chen Z, Xue Q, Chen X, Wang Y, Yan D, Chen S, Fan Y, Tang D, Yu W, Liu J, Chen X
Cell death & disease 2025 Jul 26;16(1):564

USP5-Mediated PD-L1 deubiquitination regulates immunotherapy efficacy in melanoma.

Xie J, Zhang P, Liu Y, Wu D, Ou X, Wang M, Yan W, Liu J, Tang J, Ni T, Ma C, Tang Q, Zhao S, Qi M
Journal of translational medicine 2025 Jul 10;23(1):778

The m(6)A methyltransferase METTL3 drives neuroinflammation and neurotoxicity through stabilizing BATF mRNA in microglia.

Wu X, Liu H, Wang J, Zhang S, Hu Q, Wang T, Cui W, Shi Y, Bai H, Zhou J, Han L, Li L, Zhao T, Wu Y, Luo J, Feng D, Guo W, Ge S, Qu Y
Cell death and differentiation 2025 Jan;32(1):100-117

LRP11 facilitates lipid metabolism and malignancy in hepatocellular carcinoma by stabilizing RACK1 through USP5 regulation.

Liang L, Jia W, Wang J, Feng Y, Zhu D, Zhao W, Xu C, Ling X, Lv Q, Ai X, Kong L, Ding W
Molecular medicine (Cambridge, Mass.) 2025 Jan 31;31(1):35

The UP1 domain is essential for the facilitation effect of HnRNP A1 on PRRSV-2 replication.

Jing H, Liu Y, Peng Z, Duan E, Liu J, Lv Y, Sun Y, Dong W, Li X, Wang J, Cao S, Wang H, Zhang Y, Li H
Virology 2025 Feb;603:110378

LC3-dependent intercellular transfer of phosphorylated STAT1/2 elicits CXCL9+ macrophages and enhances radiation-induced antitumor immunity.

Li JY, Li YQ, Dai JH, Gong S, He QM, Bai JW, Huang SW, Lu YQ, Duan YF, Feng SY, Tan XR, Liang XY, Ma J, Guo R, Liu N
The Journal of clinical investigation 2025 Dec 1;135(23)

METTL1-driven epitranscriptomic enhancement of TXNDC12 boosts c-Myc stability through USP5 in HNSCC.

Mai Z, Zheng J, Lu Y, Lin P, Lin Y, Zheng Y, Chen X, Guo B, Cui L, Zhao X
Experimental & molecular medicine 2025 Aug;57(8):1743-1758

USP5-Rich Apoptotic Extracellular Vesicles Regulate Nucleus Pulposus Cells Apoptosis and DNA Damage Repair by Preventing E2F1 Proteasomal Degradation.

Shi P, Gao H, Cheng Z, Wu W, Zhang A, Chen X, Wu W, Zhang Y
Journal of extracellular vesicles 2025 Aug;14(8):e70148

Synthesis of USP5-IN-1 derivatives as novel USP5 inhibitors with potent activity against cholangiocarcinoma cells.

Hu X, Yu Z, Zhu H, Cui H, Ning F, Dai J, Zhang J, Li Y, Zhang X
Bioorganic & medicinal chemistry 2025 Aug 15;126:118213

MSK1 promotes colorectal cancer metastasis by increasing Snail protein stability through USP5-mediated Snail deubiquitination.

Hong KS, Ryu KJ, Kim H, Kim M, Park SH, Kim T, Yang JW, Hwangbo C, Kim KD, Park YJ, Yoo J
Experimental & molecular medicine 2025 Apr;57(4):820-835

Adipose stem cell exosomes, stimulated by pro-inflammatory factors, enhance immune evasion in triple-negative breast cancer by modulating the HDAC6/STAT3/PD-L1 pathway through the transporter UCHL1.

Zhu Q, Zhang K, Cao Y, Hu Y
Cancer cell international 2024 Nov 20;24(1):385

USP9X-mediated REV1 deubiquitination promotes lung cancer radioresistance via the action of REV1 as a Rad18 molecular scaffold for cystathionine γ-lyase.

Chen Y, Feng X, Wu Z, Yang Y, Rao X, Meng R, Zhang S, Dong X, Xu S, Wu G, Jie X
Journal of biomedical science 2024 May 28;31(1):55

P62 promotes FSH-induced antral follicle formation by directing degradation of ubiquitinated WT1.

Zhao T, He M, Zhu Z, Zhang T, Zheng W, Qin S, Gao M, Wang W, Chen Z, Han J, Liu L, Zhou B, Wang H, Zhang H, Xia G, Wang J, Wang F, Wang C
Cellular and molecular life sciences : CMLS 2024 May 20;81(1):221

The deubiquitinase USP5 promotes cholangiocarcinoma progression by stabilizing YBX1.

Ning F, Du L, Li J, Wu T, Zhou J, Chen Z, Hu X, Zhang Y, Luan X, Xin H, Yuan C, Zhang X
Life sciences 2024 Jul 1;348:122674

USP5 facilitates bladder cancer progression by stabilizing the c-Jun protein.

Zhang HH, Zhang AQ, Peng P, Huang L, Liu CY, Nie XR, Hou DF, Zhang X, Li SZ
Cancer cell international 2024 Jan 16;24(1):32

Bruceine B Displays Potent Antimyeloma Activity by Inducing the Degradation of the Transcription Factor c-Maf.

Li H, Zhu X, Sun Z, Wang Q, Song S, Xu Y, He G, Mao X
ACS pharmacology & translational science 2024 Jan 12;7(1):176-185

PFKP deubiquitination and stabilization by USP5 activate aerobic glycolysis to promote triple-negative breast cancer progression.

Peng ZM, Han XJ, Wang T, Li JJ, Yang CX, Tou FF, Zhang Z
Breast cancer research : BCR 2024 Jan 12;26(1):10

USP5 promotes tumor progression by stabilizing SLUG in bladder cancer.

Wan QK, Li TT, Liu BB, He B
Oncology letters 2024 Dec;28(6):572

The ubiquitin ligase UBR4 and the deubiquitylase USP5 modulate the stability of DNA mismatch repair protein MLH1.

Mao C, Li S, Che J, Liu D, Mao X, Rao H
The Journal of biological chemistry 2024 Aug;300(8):107592

The ubiquitin-specific protease 5 mediated deubiquitination of LSH links metabolic regulation of ferroptosis to hepatocellular carcinoma progression.

Yan B, Guo J, Wang Z, Ning J, Wang H, Shu L, Hu K, Chen L, Shi Y, Zhang L, Liu S, Tao Y, Xiao D
MedComm 2023 Aug;4(4):e337

The deubiquitinase Leon/USP5 interacts with Atg1/ULK1 and antagonizes autophagy.

Pai YL, Lin YJ, Peng WH, Huang LT, Chou HY, Wang CH, Chien CT, Chen GC
Cell death & disease 2023 Aug 22;14(8):540

USP5 promotes lipopolysaccharide-induced apoptosis and inflammatory response by stabilizing the TXNIP protein.

Shi S, Pan X, Chen M, Zhang L, Zhang S, Wang X, Shi S, Chen Z, Lin W, Jiang Y
Hepatology communications 2023 Aug 1;7(8)

The Mechano-Ubiquitinome of Articular Cartilage: Differential Ubiquitination and Activation of a Group of ER-Associated DUBs and ER Stress Regulators.

Kaokhum N, Pinto-Fernández A, Wilkinson M, Kessler BM, Ismail HM
Molecular & cellular proteomics : MCP 2022 Dec;21(12):100419

miR-141-3p affects β-catenin signaling and apoptosis by targeting Ubtd2 in rats with anorectal malformations.

Wang CY, Li SY, Xiao YX, Zhen L, Wei XG, Tang XB, Yuan ZW, Bai YZ
Annals of the New York Academy of Sciences 2022 Dec;1518(1):315-327

STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity.

Ohishi H, Shimada S, Uchino S, Li J, Sato Y, Shintani M, Owada H, Ohkawa Y, Pertsinidis A, Yamamoto T, Kimura H, Ochiai H
Nature communications 2022 Dec 20;13(1):7672

USP5 promotes breast cancer cell proliferation and metastasis by stabilizing HIF2α.

Huang W, Liu X, Zhang Y, Deng M, Li G, Chen G, Yu L, Jin L, Liu T, Wang Y, Chen Y
Journal of cellular physiology 2022 Apr;237(4):2211-2219

Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1.

Zhang Z, Cui Z, Xie Z, Li C, Xu C, Guo X, Yu J, Chen T, Facchinetti F, Bohnenberger H, Leong TL, Xie Y, Mao X, Zhao J
Translational lung cancer research 2021 Oct;10(10):3995-4011

Ubiquitin specific peptidase 5 enhances STAT3 signaling and promotes migration and invasion in Pancreatic Cancer.

Lian J, Liu C, Guan X, Wang B, Yao Y, Su D, Ma Y, Fang L, Zhang Y
Journal of Cancer 2020;11(23):6802-6811

USP5 Promotes Metastasis in Non-Small Cell Lung Cancer by Inducing Epithelial-Mesenchymal Transition via Wnt/β-Catenin Pathway.

Xue S, Wu W, Wang Z, Lu G, Sun J, Jin X, Xie L, Wang X, Tan C, Wang Z, Wang W, Ding X
Frontiers in pharmacology 2020;11:668

MAFG-AS1 promotes tumor progression via regulation of the HuR/PTBP1 axis in bladder urothelial carcinoma.

Xiao M, Liu J, Xiang L, Zhao K, He D, Zeng Q, Zhang Q, Xie D, Deng M, Zhu Y, Zhang Y, Liu Y, Bo H, Liu X, Chen X, Gong L, Bao Y, Hu Y, Cheng Y, Deng L, Zhu R, Xing X, Zhou M, Xiong W, Zhou Y, Zhou J, Li X, Cao K
Clinical and translational medicine 2020 Dec;10(8):e241

Ca(v)3.2 overexpression in L4 dorsal root ganglion neurons after L5 spinal nerve cutting involves Egr-1, USP5 and HMGB1 in rats: An emerging signaling pathway for neuropathic pain.

Tomita S, Sekiguchi F, Kasanami Y, Naoe K, Tsubota M, Wake H, Nishibori M, Kawabata A
European journal of pharmacology 2020 Dec 5;888:173587

Ubiquitin specific peptidase 5 regulates colorectal cancer cell growth by stabilizing Tu translation elongation factor.

Xu X, Huang A, Cui X, Han K, Hou X, Wang Q, Cui L, Yang Y
Theranostics 2019;9(14):4208-4220

Mebendazole elicits potent antimyeloma activity by inhibiting the USP5/c-Maf axis.

Chen XH, Xu YJ, Wang XG, Lin P, Cao BY, Zeng YY, Wang Q, Zhang ZB, Mao XL, Zhang T
Acta pharmacologica Sinica 2019 Dec;40(12):1568-1577

SUMOylation regulates USP5-Cav3.2 calcium channel interactions.

Garcia-Caballero A, Zhang FX, Chen L, M'Dahoma S, Huang J, Zamponi GW
Molecular brain 2019 Aug 27;12(1):73

Ubiquitin-specific peptidase 5 and ovarian tumor deubiquitinase 6A are differentially expressed in p53+/+ and p53-/- HCT116 cells.

Kim SY, Kwon SK, Lee SY, Baek KH
International journal of oncology 2018 May;52(5):1705-1714

Zinc deficiency promotes cystitis-related bladder pain by enhancing function and expression of Ca(v)3.2 in mice.

Ozaki T, Matsuoka J, Tsubota M, Tomita S, Sekiguchi F, Minami T, Kawabata A
Toxicology 2018 Jan 15;393:102-112

Selective inhibition of Ca(V)3.2 channels reverses hyperexcitability of peripheral nociceptors and alleviates postsurgical pain.

Joksimovic SL, Joksimovic SM, Tesic V, García-Caballero A, Feseha S, Zamponi GW, Jevtovic-Todorovic V, Todorovic SM
Science signaling 2018 Aug 28;11(545)

Deubiquitylases USP5 and USP13 are recruited to and regulate heat-induced stress granules through their deubiquitylating activities.

Xie X, Matsumoto S, Endo A, Fukushima T, Kawahara H, Saeki Y, Komada M
Journal of cell science 2018 Apr 12;131(8)

Inhibition of the deubiquitinase USP5 leads to c-Maf protein degradation and myeloma cell apoptosis.

Wang S, Juan J, Zhang Z, Du Y, Xu Y, Tong J, Cao B, Moran MF, Zeng Y, Mao X
Cell death & disease 2017 Sep 21;8(9):e3058

USP5 promotes tumorigenesis and progression of pancreatic cancer by stabilizing FoxM1 protein.

Li XY, Wu HY, Mao XF, Jiang LX, Wang YX
Biochemical and biophysical research communications 2017 Oct 7;492(1):48-54

High glucose induces N-linked glycosylation-mediated functional upregulation and overexpression of Ca(v)3.2 T-type calcium channels in neuroendocrine-like differentiated human prostate cancer cells.

Fukami K, Asano E, Ueda M, Sekiguchi F, Yoshida S, Kawabata A
Journal of pharmacological sciences 2017 Jan;133(1):57-60

Identification of interleukin-1 beta as a key mediator in the upregulation of Cav3.2-USP5 interactions in the pain pathway.

Stemkowski PL, Garcia-Caballero A, Gadotti VM, M'Dahoma S, Chen L, Souza IA, Zamponi GW
Molecular pain 2017 Jan-Dec;13:1744806917724698

A cell-permeant peptide corresponding to the cUBP domain of USP5 reverses inflammatory and neuropathic pain.

Garcia-Caballero A, Gadotti VM, Chen L, Zamponi GW
Molecular pain 2016;12

The ubiquitin ligase HERC4 mediates c-Maf ubiquitination and delays the growth of multiple myeloma xenografts in nude mice.

Zhang Z, Tong J, Tang X, Juan J, Cao B, Hurren R, Chen G, Taylor P, Xu X, Shi CX, Du J, Hou J, Wang G, Wu D, Stewart AK, Schimmer AD, Moran MF, Mao X
Blood 2016 Mar 31;127(13):1676-86

Smurf1 represses TNF-α production through ubiquitination and destabilization of USP5.

Qian G, Ren Y, Zuo Y, Yuan Y, Zhao P, Wang X, Cheng Q, Liu J, Zhang L, Guo T, Liu C, Zheng H
Biochemical and biophysical research communications 2016 Jun 3;474(3):491-496

Small organic molecule disruptors of Cav3.2 - USP5 interactions reverse inflammatory and neuropathic pain.

Gadotti VM, Caballero AG, Berger ND, Gladding CM, Chen L, Pfeifer TA, Zamponi GW
Molecular pain 2015 Mar 14;11:12

A ubiquitin shuttle DC-UbP/UBTD2 reconciles protein ubiquitination and deubiquitination via linking UbE1 and USP5 enzymes.

Song AX, Yang H, Gao YG, Zhou CJ, Zhang YH, Hu HY
PloS one 2014;9(9):e107509

Ubiquitin-specific protease 5 is required for the efficient repair of DNA double-strand breaks.

Nakajima S, Lan L, Wei L, Hsieh CL, Rapić-Otrin V, Yasui A, Levine AS
PloS one 2014;9(1):e84899

Selenoprotein S is involved in maintenance and transport of multiprotein complexes.

Turanov AA, Shchedrina VA, Everley RA, Lobanov AV, Yim SH, Marino SM, Gygi SP, Hatfield DL, Gladyshev VN
The Biochemical journal 2014 Sep 15;462(3):555-65

Isolation of mammalian 26S proteasomes and p97/VCP complexes using the ubiquitin-like domain from HHR23B reveals novel proteasome-associated proteins.

Besche HC, Haas W, Gygi SP, Goldberg AL
Biochemistry 2009 Mar 24;48(11):2538-49

Suppression of the deubiquitinating enzyme USP5 causes the accumulation of unanchored polyubiquitin and the activation of p53.

Dayal S, Sparks A, Jacob J, Allende-Vega N, Lane DP, Saville MK
The Journal of biological chemistry 2009 Feb 20;284(8):5030-41

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The USP5 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human USP5. This antibody recognizes human, mouse, rat antigen. The USP5 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 10473-1-AP.pdf

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