Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-52414 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPINK4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LPFSRMPICE HMVESPTCSQ MSNLVCGTDG LTYTNECQLC LARIKTKQDI QIMKDGK Highest antigen sequence identity to the following orthologs: Mouse - 70%, Rat - 65%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references BMP gradient along the intestinal villus axis controls zonated enterocyte and goblet cell states.
Beumer J, Puschhof J, Yengej FY, Zhao L, Martinez-Silgado A, Blotenburg M, Begthel H, Boot C, van Oudenaarden A, Chen YG, Clevers H
Cell reports 2022 Mar 1;38(9):110438
Cell reports 2022 Mar 1;38(9):110438
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SPINK4 in human cell line HeLa shows positivity in nucleus, the Golgi apparatus & vesicles. Samples were probed using a SPINK4 Polyclonal Antibody (Product # PA5-52414).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPINK4 in human rectum and liver tissues using SPINK4 Polyclonal Antibody (Product # PA5-52414). Corresponding SPINK4 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPINK4 in human rectum using SPINK4 Polyclonal Antibody (Product # PA5-52414) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPINK4 in human liver using SPINK4 Polyclonal Antibody (Product # PA5-52414) shows low expression as expected.