Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF1747 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Trappin-2/Elafin/Skalp Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Trappin-2/Elafin in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) SLPI, rhTGM-2, and human Neutrophil Elastase is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references A systems biology examination of the human female genital tract shows compartmentalization of immune factor expression.
Anti-HIV-1 activity of elafin is more potent than its precursor's, trappin-2, in genital epithelial cells.
Elafin, an elastase-specific inhibitor, is cleaved by its cognate enzyme neutrophil elastase in sputum from individuals with cystic fibrosis.
Burgener A, Tjernlund A, Kaldensjo T, Abou M, McCorrister S, Westmacott GR, Mogk K, Ambrose E, Broliden K, Ball B
Journal of virology 2013 May;87(9):5141-50
Journal of virology 2013 May;87(9):5141-50
Anti-HIV-1 activity of elafin is more potent than its precursor's, trappin-2, in genital epithelial cells.
Drannik AG, Nag K, Yao XD, Henrick BM, Jain S, Ball TB, Plummer FA, Wachihi C, Kimani J, Rosenthal KL
Journal of virology 2012 Apr;86(8):4599-610
Journal of virology 2012 Apr;86(8):4599-610
Elafin, an elastase-specific inhibitor, is cleaved by its cognate enzyme neutrophil elastase in sputum from individuals with cystic fibrosis.
Guyot N, Butler MW, McNally P, Weldon S, Greene CM, Levine RL, O'Neill SJ, Taggart CC, McElvaney NG
The Journal of biological chemistry 2008 Nov 21;283(47):32377-85
The Journal of biological chemistry 2008 Nov 21;283(47):32377-85
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Neutralization of Trappin-2/ Elafin Activity by Human Trappin-2/Elafin Antibody. Recombinant Mouse Neutrophil Elastase/ELA2 (1 µg/mL, Catalog # 4517-SE) activity is measured in the presence of Recombinant Human Trappin-2/ Elafin (1 µg/mL, Catalog # 1747-PI) that has been pre-incubated with increasing concentrations of Goat Anti-Human Trappin-2/Elafin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1747). The ND50 is typically 15.6 µg/mL.