AP6869A

antibody from Abgent

Targeting: SPINK5 DKFZp686K19184, FLJ21544, FLJ97536, FLJ97596, FLJ99794, LEKTI, LETKI, NETS, NS, VAKTI

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP6869A

Provider

Abgent

Proper citation

Abgent Cat#AP6869a, RRID:AB_1968324

Product name

SPINK5 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of SPINK5 Antibody (N-term) (Cat. #AP6869a) in A2058 cell line lysates (35ug/lane). SPINK5 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

SPINK5 Antibody (N-term) (RB18954) IHC analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPINK5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

SPINK5 Antibody (N-term) (Cat. #AP6869a) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50