LS-C377072

antibody from LSBio

Targeting: ZNF346 JAZ, Zfp346

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C377072

Provider

LSBio

Product name

ZNF346 Antibody (aa1-294) LS-C377072

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating ZNF346 in HepG2 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of ZNF346 Antibody in HepG2 whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/50000) Lane 2: ZNF346 Antibody (4µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating ZNF346 in HepG2 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of ZNF346 Antibody in HepG2 whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/50000) Lane 2: ZNF346 Antibody (4µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating ZNF346 in HepG2 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of ZNF346 Antibody in HepG2 whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/50000) Lane 2: ZNF346 Antibody (4µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (20µg)

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsil using antibody at 1:100 dilution.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using antibody at 1:100 dilution.