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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-21773 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEC61A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: K562 whole cell extract, K562 membrane fraction extract (un-boiled).
- Concentration
- 0.15 mg/mL
Submitted references Substrate-driven assembly of a translocon for multipass membrane proteins.
An ER translocon for multi-pass membrane protein biogenesis.
Sundaram A, Yamsek M, Zhong F, Hooda Y, Hegde RS, Keenan RJ
Nature 2022 Nov;611(7934):167-172
Nature 2022 Nov;611(7934):167-172
An ER translocon for multi-pass membrane protein biogenesis.
McGilvray PT, Anghel SA, Sundaram A, Zhong F, Trnka MJ, Fuller JR, Hu H, Burlingame AL, Keenan RJ
eLife 2020 Aug 21;9
eLife 2020 Aug 21;9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Sec61 alpha-1 using 30 µg of K562 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Sec61 alpha-1 polyclonal antibody (Product # PA5-21773) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SEC61A1 was performed by separating 30 µg of K562 whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a SEC61A1 Polyclonal Antibody (Product # PA5-21773) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Sec61 alpha-1 in methanol-fixed A431 cells using a Sec61 alpha-1 polyclonal antibody (Product # PA5-21773) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed A431, using SEC61A1 (Product # PA5-21773) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SEC61A1 (Product # PA5-21773) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. Natively isolated TMCO1-ribosome complexes contain multiple transmembrane components. ( A ) Emetine- and micrococcal nuclease-treated membranes from wild-type (WT) or 3xFlag-TMCO1 (Flag) HEK293 cells were digitonin-solubilized, immunoprecipitated via the 3xFlag tag on TMCO1, and the eluate sedimented through a sucrose cushion to isolate the ribosome-associated fraction for analysis. ( B ) Proteins enriched in the ribosomal fraction after immunoprecipitation from 3xFlag-TMCO1 or wild-type membranes. ( C ) Top hits were confirmed by western blotting. The catalytic STT3A subunit of the OST complex is not detected. ( D ) Topology and domain structure for the top hits, based on Uniprot annotation, homology modeling, de novo structure prediction (in RaptorX-Contact), and experimental mapping; the Sec61 complex is not shown. Distinguishing features include the large globular luminal domain of Nicalin (in contrast with the flexible luminal domains of NOMO and CCDC47), the large globular cytosolic domain of CCDC47 (with a conserved C-terminal coiled-coil), and a conserved cytosolic coiled-coil between the first two TMDs of TMCO1. TMEM147 is the core, multi-pass subunit of the Nicalin-TMEM147-NOMO complex ; note that the short extra-membrane loops of TMEM147 make it difficult to detect by mass spectrometry. Figure 1-figure supplement 1. Additional interaction analysis of the TMCO1 translocon components. ( A ) Comparison of co-purifying components in the ribosome-bound fractio
