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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-53523 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USP30 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: IEAKGTLNGE KVEHQRTTFV KQLKLGKLPQ CLCIHLQRLS WSSHGTPLKR HEHVQFNEFL MMDIYKYHLL GHKPSQHNPK LNKNPGPTLE LQDGPGAPTP VLNQPGAPKT QIFMNGACSP SLLPTLSAPM PFPLPVVPDY SSST Highest antigen sequence identity to the following orthologs: Mouse - 83%, Rat - 81%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references USP30 sets a trigger threshold for PINK1-PARKIN amplification of mitochondrial ubiquitylation.
Rusilowicz-Jones EV, Jardine J, Kallinos A, Pinto-Fernandez A, Guenther F, Giurrandino M, Barone FG, McCarron K, Burke CJ, Murad A, Martinez A, Marcassa E, Gersch M, Buckmelter AJ, Kayser-Bricker KJ, Lamoliatte F, Gajbhiye A, Davis S, Scott HC, Murphy E, England K, Mortiboys H, Komander D, Trost M, Kessler BM, Ioannidis S, Ahlijanian MK, Urbé S, Clague MJ
Life science alliance 2020 Aug;3(8)
Life science alliance 2020 Aug;3(8)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of USP30 in control (vector only transfected HEK293T lysate) and USP30 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a USP30 Polyclonal Antibody (Product # PA5-53523).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of USP30 in human cerebral cortex using an USP30 Polyclonal Antibody (Product # PA5-53523) shows strong cytoplasmic positivity in neurons.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of USP30 in human testis using an USP30 Polyclonal Antibody (Product # PA5-53523) shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts and in Leydig cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of USP30 in human kidney using an USP30 Polyclonal Antibody (Product # PA5-53523) shows moderate to strong cytoplasmic positivity in renal tubules cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of USP30 in human liver using an USP30 Polyclonal Antibody (Product # PA5-53523) shows moderate cytoplasmic positivity in hepatocytes.
