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- Antigen structure
- References [7]
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- Western blot [1]
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- Product number
- HPA016952 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA016952, RRID:AB_1858680
- Product name
- Anti-USP30
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human USP30, Gene description: ubiquitin specific peptidase 30, Alternative Gene Names: FLJ40511, MGC10702, Validated applications: IHC, WB, Uniprot ID: Q70CQ3, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Benchmarking a highly selective USP30 inhibitor for enhancement of mitophagy and pexophagy
Investigation of USP30 inhibition to enhance Parkin-mediated mitophagy: tools and approaches
USP30 sets a trigger threshold for PINK1–PARKIN amplification of mitochondrial ubiquitylation
USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy
Dual role of USP 30 in controlling basal pexophagy and mitophagy
Mechanism and regulation of the Lys6-selective deubiquitinase USP30.
The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy
Rusilowicz-Jones E, Barone F, Lopes F, Stephen E, Mortiboys H, Urbé S, Clague M
Life Science Alliance 2021;5(2):e202101287
Life Science Alliance 2021;5(2):e202101287
Investigation of USP30 inhibition to enhance Parkin-mediated mitophagy: tools and approaches
Tsefou E, Walker A, Clark E, Hicks A, Luft C, Takeda K, Watanabe T, Ramazio B, Staddon J, Briston T, Ketteler R
Biochemical Journal 2021;478(23):4099-4118
Biochemical Journal 2021;478(23):4099-4118
USP30 sets a trigger threshold for PINK1–PARKIN amplification of mitochondrial ubiquitylation
Rusilowicz-Jones E, Jardine J, Kallinos A, Pinto-Fernandez A, Guenther F, Giurrandino M, Barone F, McCarron K, Burke C, Murad A, Martinez A, Marcassa E, Gersch M, Buckmelter A, Kayser-Bricker K, Lamoliatte F, Gajbhiye A, Davis S, Scott H, Murphy E, England K, Mortiboys H, Komander D, Trost M, Kessler B, Ioannidis S, Ahlijanian M, Urbé S, Clague M
Life Science Alliance 2020;3(8):e202000768
Life Science Alliance 2020;3(8):e202000768
USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy
Niu K, Fang H, Chen Z, Zhu Y, Tan Q, Wei D, Li Y, Balajee A, Zhao Y
Autophagy 2019;16(4):724-734
Autophagy 2019;16(4):724-734
Dual role of USP 30 in controlling basal pexophagy and mitophagy
Marcassa E, Kallinos A, Jardine J, Rusilowicz‐Jones E, Martinez A, Kuehl S, Islinger M, Clague M, Urbé S
EMBO reports 2018;19(7)
EMBO reports 2018;19(7)
Mechanism and regulation of the Lys6-selective deubiquitinase USP30.
Gersch M, Gladkova C, Schubert AF, Michel MA, Maslen S, Komander D
Nature structural & molecular biology 2017 Nov;24(11):920-930
Nature structural & molecular biology 2017 Nov;24(11):920-930
The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy
Bingol B, Tea J, Phu L, Reichelt M, Bakalarski C, Song Q, Foreman O, Kirkpatrick D, Sheng M
Nature 2014;510(7505):370-375
Nature 2014;510(7505):370-375
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and USP30 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409983).
- Sample type
- Human
- Protocol
- Protocol
