10210-2-AP

antibody from Proteintech Group

Targeting: RUVBL1 ECP54, INO80H, NMP238, Pontin52, RVB1, TIH1, TIP49, TIP49a

Provider product page for 10210-2-AP

Western blot

ELISA

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [32]
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Validations
Western blot [1]
Immunoprecipitation [1]

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Product number: 10210-2-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10210-2-AP, RRID:AB_2184405
Product name: RUVBL1 antibody
Antibody type: Polyclonal
Description: KD/KO validated RUVBL1 antibody (Cat. #10210-2-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

RUVBL1 protein structure - 10210-2-AP shown in red.

Submitted references NOPCHAP1 is a PAQosome cofactor that helps loading NOP58 on RUVBL1/2 during box C/D snoRNP biogenesis.

Abel Y, Paiva ACF, Bizarro J, Chagot ME, Santo PE, Robert MC, Quinternet M, Vandermoere F, Sousa PMF, Fort P, Charpentier B, Manival X, Bandeiras TM, Bertrand E, Verheggen C
Nucleic acids research 2021 Jan 25;49(2):1094-1113

Targeted deletion of Ruvbl1 results in severe defects of epidermal development and perinatal mortality.

Dafinger C, Benzing T, Dötsch J, Schermer B, Liebau MC
Molecular and cellular pediatrics 2021 Feb 12;8(1):1

Linc-MYH configures INO80 to regulate muscle stem cell numbers and skeletal muscle hypertrophy.

Schutt C, Hallmann A, Hachim S, Klockner I, Valussi M, Atzberger A, Graumann J, Braun T, Boettger T
The EMBO journal 2020 Nov 16;39(22):e105098

Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.

Shin SH, Lee JS, Zhang JM, Choi S, Boskovic ZV, Zhao R, Song M, Wang R, Tian J, Lee MH, Kim JH, Jeong M, Lee JH, Petukhov M, Lee SW, Kim SG, Zou L, Byun S
Science advances 2020 Jul;6(31):eaay9131

Assembly of the asymmetric human γ-tubulin ring complex by RUVBL1-RUVBL2 AAA ATPase.

Zimmermann F, Serna M, Ezquerra A, Fernandez-Leiro R, Llorca O, Luders J
Science advances 2020 Dec;6(51)

Functional genetic variants of RUVBL1 predict overall survival of Chinese patients with epithelial ovarian cancer.

Li H, Tong X, Xu Y, Wang M, Dai H, Shi T, Sun M, Chen K, Cheng X, Wei Q
Carcinogenesis 2019 Oct 16;40(10):1209-1219

RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome.

Trotman JB, Agana BA, Giltmier AJ, Wysocki VH, Schoenberg DR
The Journal of biological chemistry 2018 Oct 26;293(43):16596-16607

Targeted deletion of the AAA-ATPase Ruvbl1 in mice disrupts ciliary integrity and causes renal disease and hydrocephalus.

Dafinger C, Rinschen MM, Borgal L, Ehrenberg C, Basten SG, Franke M, Höhne M, Rauh M, Göbel H, Bloch W, Wunderlich FT, Peters DJM, Tasche D, Mishra T, Habbig S, Dötsch J, Müller RU, Brüning JC, Persigehl T, Giles RH, Benzing T, Schermer B, Liebau MC
Experimental & molecular medicine 2018 Jun 28;50(6):1-17

SAM68 is required for regulation of Pumilio by the NORAD long noncoding RNA.

Tichon A, Perry RB, Stojic L, Ulitsky I
Genes & development 2018 Jan 1;32(1):70-78

Revealing A-Raf functions through its interactome.

Zhang XY, Guo H, Han B, Zhang XM, Huang Y, Yang Y, Liu Y, Guo XX, Hao Q, An S, Xu TR
Biochimica et biophysica acta. Proteins and proteomics 2018 Aug;1866(8):849-856

RUVBL1, a novel C-RAF-binding protein, activates the RAF/MEK/ERK pathway to promote lung cancer tumorigenesis.

Guo H, Zhang XY, Peng J, Huang Y, Yang Y, Liu Y, Guo XX, Hao Q, An S, Xu TR
Biochemical and biophysical research communications 2018 Apr 15;498(4):932-939

Decoding the full picture of Raf1 function based on its interacting proteins.

Huang Y, Guo XX, Han B, Zhang XM, An S, Zhang XY, Yang Y, Liu Y, Hao Q, Xu TR
Oncotarget 2017 Sep 15;8(40):68329-68337

Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones.

Malinová A, Cvačková Z, Matějů D, Hořejší Z, Abéza C, Vandermoere F, Bertrand E, Staněk D, Verheggen C
The Journal of cell biology 2017 Jun 5;216(6):1579-1596

RUVBL1-ITFG1 interaction is required for collective invasion in breast cancer.

Fan W, Xie J, Xia J, Zhang Y, Yang M, Wang H, Pan Y, Zhang M, Han B, Wu B, Hou Z, Liang D, Wang C, Xu J, Song L, Liu Q
Biochimica et biophysica acta. General subjects 2017 Jul;1861(7):1788-1800

Reptin regulates insulin-stimulated Akt phosphorylation in hepatocellular carcinoma via the regulation of SHP-1/PTPN6.

Raymond AA, Javary J, Breig O, Neaud V, Rosenbaum J
Cell biochemistry and function 2017 Aug;35(6):289-295

Specific threonine-4 phosphorylation and function of RNA polymerase II CTD during M phase progression.

Hintermair C, Voß K, Forné I, Heidemann M, Flatley A, Kremmer E, Imhof A, Eick D
Scientific reports 2016 Jun 6;6:27401

R loops regulate promoter-proximal chromatin architecture and cellular differentiation.

Chen PB, Chen HV, Acharya D, Rando OJ, Fazzio TG
Nature structural & molecular biology 2015 Dec;22(12):999-1007

An inherited immunoglobulin class-switch recombination deficiency associated with a defect in the INO80 chromatin remodeling complex.

Kracker S, Di Virgilio M, Schwartzentruber J, Cuenin C, Forveille M, Deau MC, McBride KM, Majewski J, Gazumyan A, Seneviratne S, Grimbacher B, Kutukculer N, Herceg Z, Cavazzana M, Jabado N, Nussenzweig MC, Fischer A, Durandy A
The Journal of allergy and clinical immunology 2015 Apr;135(4):998-1007.e6

Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control.

Bizarro J, Charron C, Boulon S, Westman B, Pradet-Balade B, Vandermoere F, Chagot ME, Hallais M, Ahmad Y, Leonhardt H, Lamond A, Manival X, Branlant C, Charpentier B, Verheggen C, Bertrand E
The Journal of cell biology 2014 Nov 24;207(4):463-80

LINKIN, a new transmembrane protein necessary for cell adhesion.

Kato M, Chou TF, Yu CZ, DeModena J, Sternberg PW
eLife 2014 Dec 1;3:e04449

Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex.

Kim SG, Hoffman GR, Poulogiannis G, Buel GR, Jang YJ, Lee KW, Kim BY, Erikson RL, Cantley LC, Choo AY, Blenis J
Molecular cell 2013 Jan 10;49(1):172-85

The ATPase activity of reptin is required for its effects on tumor cell growth and viability in hepatocellular carcinoma.

Grigoletto A, Neaud V, Allain-Courtois N, Lestienne P, Rosenbaum J
Molecular cancer research : MCR 2013 Feb;11(2):133-9

Mechanism of the AAA+ ATPases pontin and reptin in the biogenesis of H/ACA RNPs.

Machado-Pinilla R, Liger D, Leulliot N, Meier UT
RNA (New York, N.Y.) 2012 Oct;18(10):1833-45

Quantitative analysis of the chromatin proteome in disease reveals remodeling principles and identifies high mobility group protein B2 as a regulator of hypertrophic growth.

Franklin S, Chen H, Mitchell-Jordan S, Ren S, Wang Y, Vondriska TM
Molecular & cellular proteomics : MCP 2012 Jun;11(6):M111.014258

A member of the ETS family, EHF, and the ATPase RUVBL1 inhibit p53-mediated apoptosis.

Taniue K, Oda T, Hayashi T, Okuno M, Akiyama T
EMBO reports 2011 Jul 1;12(7):682-9

Cooperative signaling between Wnt1 and integrin-linked kinase induces accelerated breast tumor development.

Oloumi A, Maidan M, Lock FE, Tearle H, McKinney S, Muller WJ, Aparicio SA, Dedhar S
Breast cancer research : BCR 2010;12(3):R38

Adenosine triphosphatase pontin is overexpressed in hepatocellular carcinoma and coregulated with reptin through a new posttranslational mechanism.

Haurie V, Ménard L, Nicou A, Touriol C, Metzler P, Fernandez J, Taras D, Lestienne P, Balabaud C, Bioulac-Sage P, Prats H, Zucman-Rossi J, Rosenbaum J
Hepatology (Baltimore, Md.) 2009 Dec;50(6):1871-83

Profiling protein markers associated with the sensitivity to concurrent chemoradiotherapy in human cervical carcinoma.

Zhu H, Pei HP, Zeng S, Chen J, Shen LF, Zhong MZ, Yao RJ, Shen H
Journal of proteome research 2009 Aug;8(8):3969-76

CUL2 is required for the activity of hypoxia-inducible factor and vasculogenesis.

Maeda Y, Suzuki T, Pan X, Chen G, Pan S, Bartman T, Whitsett JA
The Journal of biological chemistry 2008 Jun 6;283(23):16084-92

Integrin-linked kinase localizes to the centrosome and regulates mitotic spindle organization.

Fielding AB, Dobreva I, McDonald PC, Foster LJ, Dedhar S
The Journal of cell biology 2008 Feb 25;180(4):681-9

The Hsp90 chaperone controls the biogenesis of L7Ae RNPs through conserved machinery.

Boulon S, Marmier-Gourrier N, Pradet-Balade B, Wurth L, Verheggen C, Jády BE, Rothé B, Pescia C, Robert MC, Kiss T, Bardoni B, Krol A, Branlant C, Allmang C, Bertrand E, Charpentier B
The Journal of cell biology 2008 Feb 11;180(3):579-95

Mapping the integrin-linked kinase interactome using SILAC.

Dobreva I, Fielding A, Foster LJ, Dedhar S
Journal of proteome research 2008 Apr;7(4):1740-9

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 10210-2-AP(RUVBL1 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP result of anti-RUVBL1(10210-2-AP for IP and Detection).
Sample type: cell line