10210-2-AP

antibody from Proteintech Group

Targeting: RUVBL1 ECP54, INO80H, NMP238, Pontin52, RVB1, TIH1, TIP49, TIP49a

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

10210-2-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10210-2-AP, RRID:AB_2184405

Product name

RUVBL1 antibody

Antibody type

Polyclonal

Description

KD/KO validated RUVBL1 antibody (Cat. #10210-2-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

The DNA helicase FANCJ (BRIP1) functions in double strand break repair processing, but not crossover formation during prophase I of meiosis in male mice.

Horan TS, Ascenção CFR, Mellor C, Wang M, Smolka MB, Cohen PE
PLoS genetics 2024 Feb;20(2):e1011175

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Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis.

Fu Y, Li L, Zhang X, Deng Z, Wu Y, Chen W, Liu Y, He S, Wang J, Xie Y, Tu Z, Lyu Y, Wei Y, Wang S, Cui CP, Liu CH, Zhang L
Nature communications 2024 Apr 6;15(1):2974

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GART Functions as a Novel Methyltransferase in the RUVBL1/β-Catenin Signaling Pathway to Promote Tumor Stemness in Colorectal Cancer.

Tang C, Ke M, Yu X, Sun S, Luo X, Liu X, Zhou Y, Wang Z, Cui X, Gu C, Yang Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Sep;10(25):e2301264

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The DNA helicase FANCJ (BRIP1) functions in Double Strand Break repair processing, but not crossover formation during Prophase I of meiosis in male mice.

Horan TS, Ascenção CFR, Mellor CA, Wang M, Smolka MB, Cohen PE
bioRxiv : the preprint server for biology 2023 Oct 8;

---

Systematic analysis of various RNA transcripts and construction of biological regulatory networks at the post-transcriptional level for chronic obstructive pulmonary disease.

Li B, Zhang J, Dong H, Feng X, Yu L, Zhu J, Zhang J
Journal of translational medicine 2023 Nov 7;21(1):790

---

Ubiquitin-specific peptidase 14 maintains estrogen receptor α stability via its deubiquitination activity in endometrial cancer.

Su Y, Zeng K, Liu S, Wu Y, Wang C, Wang S, Lin L, Zou R, Sun G, Luan R, Zhou B, Bai Y, Niu J, Zhang Y, Zhao Y
The Journal of biological chemistry 2023 Jan;299(1):102734

---

Identification of a Novel Interaction between Theileria Prohibitin (TaPHB-1) and Bovine RuvB-Like AAA ATPase 1.

Araveti PB, Kar PP, Kuriakose A, Sanju A, Kumar KA, Srivastava A
Microbiology spectrum 2023 Feb 14;11(1):e0250222

---

LINKIN-associated proteins necessary for tissue integrity during collective cell migration.

Tan CH, Cheng KW, Park H, Chou TF, Sternberg PW
bioRxiv : the preprint server for biology 2023 Aug 4;

---

Genome-scale mapping of DNA damage suppressors through phenotypic CRISPR-Cas9 screens.

Zhao Y, Tabet D, Rubio Contreras D, Lao L, Kousholt AN, Weile J, Melo H, Hoeg L, Feng S, Coté AG, Lin ZY, Setiaputra D, Jonkers J, Gingras AC, Gómez Herreros F, Roth FP, Durocher D
Molecular cell 2023 Aug 3;83(15):2792-2809.e9

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Combined CRISPRi and proteomics screening reveal a cohesin-CTCF-bound allele contributing to increased expression of RUVBL1 and prostate cancer progression.

Tian Y, Dong D, Wang Z, Wu L, Park JY, PRACTICAL consortium, Wei GH, Wang L
American journal of human genetics 2023 Aug 3;110(8):1289-1303

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Hippocalcin-Like 1 blunts liver lipid metabolism to suppress tumorigenesis via directly targeting RUVBL1-mTOR signaling.

Chen T, Yuan Z, Lei Z, Duan J, Xue J, Lu T, Yan G, Zhang L, Liu Y, Li Q, Zhang Y
Theranostics 2022;12(17):7450-7464

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RUVBL1 promotes enzalutamide resistance of prostate tumors through the PLXNA1-CRAF-MAPK pathway.

Sun F, Wang X, Hu J, Liu J, Wang X, Jia W, Yu Z, Gao L, Dou B, Zhao R, Feng T, Wang X, Zhang W, Liu H, Liu K, Shao Y, Dong X, Han B
Oncogene 2022 Jun;41(23):3239-3250

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Hierarchical regulation of the resting and activated T cell epigenome by major transcription factor families.

Zhong Y, Walker SK, Pritykin Y, Leslie CS, Rudensky AY, van der Veeken J
Nature immunology 2022 Jan;23(1):122-134

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NOPCHAP1 is a PAQosome cofactor that helps loading NOP58 on RUVBL1/2 during box C/D snoRNP biogenesis.

Abel Y, Paiva ACF, Bizarro J, Chagot ME, Santo PE, Robert MC, Quinternet M, Vandermoere F, Sousa PMF, Fort P, Charpentier B, Manival X, Bandeiras TM, Bertrand E, Verheggen C
Nucleic acids research 2021 Jan 25;49(2):1094-1113

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Targeted deletion of Ruvbl1 results in severe defects of epidermal development and perinatal mortality.

Dafinger C, Benzing T, Dötsch J, Schermer B, Liebau MC
Molecular and cellular pediatrics 2021 Feb 12;8(1):1

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Linc-MYH configures INO80 to regulate muscle stem cell numbers and skeletal muscle hypertrophy.

Schutt C, Hallmann A, Hachim S, Klockner I, Valussi M, Atzberger A, Graumann J, Braun T, Boettger T
The EMBO journal 2020 Nov 16;39(22):e105098

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Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.

Shin SH, Lee JS, Zhang JM, Choi S, Boskovic ZV, Zhao R, Song M, Wang R, Tian J, Lee MH, Kim JH, Jeong M, Lee JH, Petukhov M, Lee SW, Kim SG, Zou L, Byun S
Science advances 2020 Jul;6(31):eaay9131

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Assembly of the asymmetric human γ-tubulin ring complex by RUVBL1-RUVBL2 AAA ATPase.

Zimmermann F, Serna M, Ezquerra A, Fernandez-Leiro R, Llorca O, Luders J
Science advances 2020 Dec;6(51)

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LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.

Wang Y, Zhu P, Luo J, Wang J, Liu Z, Wu W, Du Y, Ye B, Wang D, He L, Ren W, Wang J, Sun X, Chen R, Tian Y, Fan Z
The EMBO journal 2019 Sep 2;38(17):e101110

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Functional genetic variants of RUVBL1 predict overall survival of Chinese patients with epithelial ovarian cancer.

Li H, Tong X, Xu Y, Wang M, Dai H, Shi T, Sun M, Chen K, Cheng X, Wei Q
Carcinogenesis 2019 Oct 16;40(10):1209-1219

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RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome.

Trotman JB, Agana BA, Giltmier AJ, Wysocki VH, Schoenberg DR
The Journal of biological chemistry 2018 Oct 26;293(43):16596-16607

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Targeted deletion of the AAA-ATPase Ruvbl1 in mice disrupts ciliary integrity and causes renal disease and hydrocephalus.

Dafinger C, Rinschen MM, Borgal L, Ehrenberg C, Basten SG, Franke M, Höhne M, Rauh M, Göbel H, Bloch W, Wunderlich FT, Peters DJM, Tasche D, Mishra T, Habbig S, Dötsch J, Müller RU, Brüning JC, Persigehl T, Giles RH, Benzing T, Schermer B, Liebau MC
Experimental & molecular medicine 2018 Jun 28;50(6):1-17

---

SAM68 is required for regulation of Pumilio by the NORAD long noncoding RNA.

Tichon A, Perry RB, Stojic L, Ulitsky I
Genes & development 2018 Jan 1;32(1):70-78

---

Revealing A-Raf functions through its interactome.

Zhang XY, Guo H, Han B, Zhang XM, Huang Y, Yang Y, Liu Y, Guo XX, Hao Q, An S, Xu TR
Biochimica et biophysica acta. Proteins and proteomics 2018 Aug;1866(8):849-856

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RUVBL1, a novel C-RAF-binding protein, activates the RAF/MEK/ERK pathway to promote lung cancer tumorigenesis.

Guo H, Zhang XY, Peng J, Huang Y, Yang Y, Liu Y, Guo XX, Hao Q, An S, Xu TR
Biochemical and biophysical research communications 2018 Apr 15;498(4):932-939

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Decoding the full picture of Raf1 function based on its interacting proteins.

Huang Y, Guo XX, Han B, Zhang XM, An S, Zhang XY, Yang Y, Liu Y, Hao Q, Xu TR
Oncotarget 2017 Sep 15;8(40):68329-68337

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Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones.

Malinová A, Cvačková Z, Matějů D, Hořejší Z, Abéza C, Vandermoere F, Bertrand E, Staněk D, Verheggen C
The Journal of cell biology 2017 Jun 5;216(6):1579-1596

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RUVBL1-ITFG1 interaction is required for collective invasion in breast cancer.

Fan W, Xie J, Xia J, Zhang Y, Yang M, Wang H, Pan Y, Zhang M, Han B, Wu B, Hou Z, Liang D, Wang C, Xu J, Song L, Liu Q
Biochimica et biophysica acta. General subjects 2017 Jul;1861(7):1788-1800

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Reptin regulates insulin-stimulated Akt phosphorylation in hepatocellular carcinoma via the regulation of SHP-1/PTPN6.

Raymond AA, Javary J, Breig O, Neaud V, Rosenbaum J
Cell biochemistry and function 2017 Aug;35(6):289-295

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Specific threonine-4 phosphorylation and function of RNA polymerase II CTD during M phase progression.

Hintermair C, Voß K, Forné I, Heidemann M, Flatley A, Kremmer E, Imhof A, Eick D
Scientific reports 2016 Jun 6;6:27401

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R loops regulate promoter-proximal chromatin architecture and cellular differentiation.

Chen PB, Chen HV, Acharya D, Rando OJ, Fazzio TG
Nature structural & molecular biology 2015 Dec;22(12):999-1007

---

An inherited immunoglobulin class-switch recombination deficiency associated with a defect in the INO80 chromatin remodeling complex.

Kracker S, Di Virgilio M, Schwartzentruber J, Cuenin C, Forveille M, Deau MC, McBride KM, Majewski J, Gazumyan A, Seneviratne S, Grimbacher B, Kutukculer N, Herceg Z, Cavazzana M, Jabado N, Nussenzweig MC, Fischer A, Durandy A
The Journal of allergy and clinical immunology 2015 Apr;135(4):998-1007.e6

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Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control.

Bizarro J, Charron C, Boulon S, Westman B, Pradet-Balade B, Vandermoere F, Chagot ME, Hallais M, Ahmad Y, Leonhardt H, Lamond A, Manival X, Branlant C, Charpentier B, Verheggen C, Bertrand E
The Journal of cell biology 2014 Nov 24;207(4):463-80

---

LINKIN, a new transmembrane protein necessary for cell adhesion.

Kato M, Chou TF, Yu CZ, DeModena J, Sternberg PW
eLife 2014 Dec 1;3:e04449

---

Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex.

Kim SG, Hoffman GR, Poulogiannis G, Buel GR, Jang YJ, Lee KW, Kim BY, Erikson RL, Cantley LC, Choo AY, Blenis J
Molecular cell 2013 Jan 10;49(1):172-85

---

The ATPase activity of reptin is required for its effects on tumor cell growth and viability in hepatocellular carcinoma.

Grigoletto A, Neaud V, Allain-Courtois N, Lestienne P, Rosenbaum J
Molecular cancer research : MCR 2013 Feb;11(2):133-9

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Mechanism of the AAA+ ATPases pontin and reptin in the biogenesis of H/ACA RNPs.

Machado-Pinilla R, Liger D, Leulliot N, Meier UT
RNA (New York, N.Y.) 2012 Oct;18(10):1833-45

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Quantitative analysis of the chromatin proteome in disease reveals remodeling principles and identifies high mobility group protein B2 as a regulator of hypertrophic growth.

Franklin S, Chen H, Mitchell-Jordan S, Ren S, Wang Y, Vondriska TM
Molecular & cellular proteomics : MCP 2012 Jun;11(6):M111.014258

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A member of the ETS family, EHF, and the ATPase RUVBL1 inhibit p53-mediated apoptosis.

Taniue K, Oda T, Hayashi T, Okuno M, Akiyama T
EMBO reports 2011 Jul 1;12(7):682-9

---

Cooperative signaling between Wnt1 and integrin-linked kinase induces accelerated breast tumor development.

Oloumi A, Maidan M, Lock FE, Tearle H, McKinney S, Muller WJ, Aparicio SA, Dedhar S
Breast cancer research : BCR 2010;12(3):R38

---

Adenosine triphosphatase pontin is overexpressed in hepatocellular carcinoma and coregulated with reptin through a new posttranslational mechanism.

Haurie V, Ménard L, Nicou A, Touriol C, Metzler P, Fernandez J, Taras D, Lestienne P, Balabaud C, Bioulac-Sage P, Prats H, Zucman-Rossi J, Rosenbaum J
Hepatology (Baltimore, Md.) 2009 Dec;50(6):1871-83

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Profiling protein markers associated with the sensitivity to concurrent chemoradiotherapy in human cervical carcinoma.

Zhu H, Pei HP, Zeng S, Chen J, Shen LF, Zhong MZ, Yao RJ, Shen H
Journal of proteome research 2009 Aug;8(8):3969-76

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CUL2 is required for the activity of hypoxia-inducible factor and vasculogenesis.

Maeda Y, Suzuki T, Pan X, Chen G, Pan S, Bartman T, Whitsett JA
The Journal of biological chemistry 2008 Jun 6;283(23):16084-92

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Integrin-linked kinase localizes to the centrosome and regulates mitotic spindle organization.

Fielding AB, Dobreva I, McDonald PC, Foster LJ, Dedhar S
The Journal of cell biology 2008 Feb 25;180(4):681-9

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The Hsp90 chaperone controls the biogenesis of L7Ae RNPs through conserved machinery.

Boulon S, Marmier-Gourrier N, Pradet-Balade B, Wurth L, Verheggen C, Jády BE, Rothé B, Pescia C, Robert MC, Kiss T, Bardoni B, Krol A, Branlant C, Allmang C, Bertrand E, Charpentier B
The Journal of cell biology 2008 Feb 11;180(3):579-95

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Mapping the integrin-linked kinase interactome using SILAC.

Dobreva I, Fielding A, Foster LJ, Dedhar S
Journal of proteome research 2008 Apr;7(4):1740-9

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10210-2-AP(RUVBL1 antibody) at dilution of 1:1000

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP result of anti-RUVBL1(10210-2-AP for IP and Detection).

Sample type

cell line