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LearnGTX111294
antibody from GeneTex
Targeting: RUVBL1
ECP54, INO80H, NMP238, Pontin52, RVB1, TIH1, TIP49, TIP49a
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- GTX111294 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111294, RRID:AB_11167321
- Product name
- RUVBL1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 10% SDS PAGE GTX111294 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (GTX111294) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RUVBL1 protein stained by RUVBL1 antibody (GTX111294) diluted at 1:500.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RUVBL1 protein stained by RUVBL1 antibody (GTX111294) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of RUVBL protein from Jurkat whole cell extracts using 5 £gg of RUVBL1 antibody (GTX111294).Western blot analysis was performed using RUVBL1 antibody (GTX111294).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RUVBL1(GTX111294) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus in rat heart by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. RUVBL1 antibody (GTX111294) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. RUVBL1 antibody (GTX111294) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RUVBL1 antibody detects RUVBL1 protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. RUVBL1 antibody (GTX111294) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RUVBL1 antibody detects RUVBL1 protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. RUVBL1 antibody (GTX111294) diluted at 1:500.
