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LearnH00008607-M01
antibody from Novus Biologicals
Targeting: RUVBL1
ECP54, INO80H, NMP238, Pontin52, RVB1, TIH1, TIP49, TIP49a
Western blot
Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00008607-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00008607-M01, RRID:AB_2285602
- Product name
- Mouse Monoclonal RUVBL1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RUVBL1 - RuvB-like 1 (E. coli)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The Relationship between RUVBL1 (Pontin, TIP49, NMP238) and BCL6 in Benign and Malignant Human Lymphoid Tissues.
Autoantibodies to RuvBL1 and RuvBL2: a novel systemic sclerosis-related antibody associated with diffuse cutaneous and skeletal muscle involvement.
Baron BW, Baron RM, Baron JM
Biochemistry and biophysics reports 2016 Jul;6:1-8
Biochemistry and biophysics reports 2016 Jul;6:1-8
Autoantibodies to RuvBL1 and RuvBL2: a novel systemic sclerosis-related antibody associated with diffuse cutaneous and skeletal muscle involvement.
Kaji K, Fertig N, Medsger TA Jr, Satoh T, Hoshino K, Hamaguchi Y, Hasegawa M, Lucas M, Schnure A, Ogawa F, Sato S, Takehara K, Fujimoto M, Kuwana M
Arthritis care & research 2014 Apr;66(4):575-84
Arthritis care & research 2014 Apr;66(4):575-84
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RUVBL1 Antibody (3G4-1F8) [H00008607-M01] - Analysis of RUVBL1 expression in transfected 293T cell line by RUVBL1 monoclonal antibody (M01), clone 3G4-1F8.Lane 1: RUVBL1 transfected lysate (Predicted MW: 50.2 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RUVBL1 Antibody (3G4-1F8) [H00008607-M01] - RUVBL1 monoclonal antibody (M01), clone 3G4-1F8. Analysis of RUVBL1 expression in human pancreas.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: RUVBL1 Antibody (3G4-1F8) [H00008607-M01] - Detection limit for recombinant GST tagged RUVBL1 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: RUVBL1 Antibody (3G4-1F8) [H00008607-M01] - Analysis of monoclonal antibody to RUVBL1 on HeLa cell . Antibody concentration 10 ug/ml.
