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antibody from Invitrogen Antibodies
Targeting: RUVBL1
ECP54, INO80H, NMP238, Pontin52, RVB1, TIH1, TIP49, TIP49a
Western blot
ELISA
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-80982 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-RUVBL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human RUVBL1/RVB1.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RUVBL1 Polyclonal Antibody (Product # PA5-80982) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate, Lane C: MCF7 Whole Cell Lysate, Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 55 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human RUVBL1 in human testis with RUVBL1 Polyclonal Antibody (Product # PA5-80982, 1:50,000, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RUVBL1 Immunoprecipitation using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate 2 µL with RUVBL1 Polyclonal Antibody (Product # PA5-80982) and 15 µL of 50 % Protein G agarose. Primary antibody: RUVBL1 Polyclonal Antibody, at 1:200 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1,000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 55 kDa.
