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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF8538 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal NrCAM Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse and rat NrCAM in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant human NrCAM is observed.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- 12 months from date of receipt, -20 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after opening. 6 months, -20 degreesC under sterile conditions after opening.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Mouse and Rat NrCAM by Western Blot. Western blot shows lysates of mouse brain (cerebellum) tissue and rat brain tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse/Rat NrCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8538) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for NrCAM at approximately 104-145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
