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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
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- Product number
- PA5-109308 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NRCAM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: SH-SY5Y, A549, SKOV-3, rat brain tissue, mouse brain tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NrCAM in SH-SY5Y cell lysate. Samples were incubated with NrCAM polyclonal antibody (Product # PA5-109308), at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NrCAM in SKOV-3 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NrCAM polyclonal antibody (Product # PA5-109308), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NrCAM in SH-SY5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NrCAM polyclonal antibody (Product # PA5-109308), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NrCAM in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NrCAM polyclonal antibody (Product # PA5-109308), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NrCAM in paraffin-embedded rat brain tissue. Samples were incubated with NrCAM polyclonal antibody (Product # PA5-109308), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NrCAM in paraffin-embedded mouse brain tissue. Samples were incubated with NrCAM polyclonal antibody (Product # PA5-109308), and followed by hematoxylin.
