PA5-21589

antibody from Invitrogen Antibodies

Targeting: USP15 KIAA0529, UNPH4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21589

Provider

Invitrogen Antibodies

Product name

USP15 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: K562, NIH-3T3, JC, BCL-1.

Concentration

0.3 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of USP15 using 30 µg of MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a USP15 polyclonal antibody (Product # PA5-21589) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using USP15 Polyclonal Antibody (Product # PA5-21589). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with USP15 Polyclonal Antibody (Product # PA5-21589) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of USP15 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a USP15 Polyclonal Antibody (Product # PA5-21589) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using USP15 Polyclonal Antibody (Product # PA5-21589). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC. Lane C: BCL-1. 7.5% SDS PAGE. USP15 Polyclonal Antibody (Product # PA5-21589) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using USP15 Polyclonal Antibody (Product # PA5-21589). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with USP15 Polyclonal Antibody (Product # PA5-21589) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of USP15 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: USP15 Polyclonal Antibody (Product # PA5-21589) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using USP15 (Product # PA5-21589) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.