H00055602-M06

antibody from Abnova Corporation

Targeting: CDKN2AIP CARF, FLJ20036

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00055602-M06

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00055602-M06, RRID:AB_490022

Product name

CARF monoclonal antibody (M06), clone 1F7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant CARF.

Antigen sequence

MAQEVSEYLSQNPRVAAWVEALRCDGETDKHWRHR
RDFLLRNAGDLAPAGGAASASTDEAADAESGTRNR
QLQQLISFSMAWANHVFLGCRYPQKVMDKILSMAE
GIKVTDAPTYTTRDELVAKVKKRGISSSNEGVEEP
SKKRVIEGKNSSAVEQDHAKTSAKTERASAQQENS
STCIGSAIKSESGNSARSSGISSQNSSTSDGDRSV
SSQSSSSVSSQVTTAGSGKASEAEAPDKHGSSFVS
LLKSSVNSHMTQSTDSRQQSGSPKKSALEGSSASA
SQSSSEIEVPLLGSSGSSEVELPLLSSKPSSETAS
SGLTSKTSSEASVSSSVAKNSSSSGTSLLTPKSSS
STNTSLLTSKSTSQVAASLLASKSSSQTSGSLVSK
STSLASVSQLASKSSSQTSTSQLPSKSTSQSSESS
VKFSCKLTNEDVKQKQPFFNRLYKTVAWKLVAVGG
FSPNVNHGELLNAAIEALKATLDVFFVPLKELADL
PQNKSSQESIVCELRCKSVYLGTGCGKSKENAKAV
ASREALKLFLKKKVVVKICKRKYRGSEIEDLVLLD
EESRPVNLPPALKHPQELL


Isotype

IgG

Antibody clone number

1F7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CARF expression in transfected 293T cell line by CARF monoclonal antibody (M06), clone 1F7.Lane 1: CARF transfected lysate(61.1 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CARF is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol