Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- H00055602-M06 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00055602-M06, RRID:AB_537422
- Product name
- Mouse Monoclonal CARF/CDKN2AIP Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CARF - collaborates/cooperates with ARF (alternate reading frame) protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CARF/CDKN2AIP Antibody (1F7) [H00055602-M06] - Western blot analysis of CARF over-expressed 293 cell line. Lane 2 cotransfected with CARF Validated Chimera RNAi or non-transfected control. Blot probed with H00055602-M06. GAPDH ( 36.1 kDa ) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CARF/CDKN2AIP Antibody (1F7) [H00055602-M06] - Analysis of CARF expression in transfected 293T cell line by CARF monoclonal antibody (M06), clone 1F7. Lane 1: CARF transfected lysatE (61.1 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: CARF/CDKN2AIP Antibody (1F7) [H00055602-M06] - Detection limit for recombinant GST tagged CARF is approximately 0.1ng/ml as a capture antibody.