IC4027G-100UG

antibody from R&D Systems

Targeting: MYF5 bHLHc2

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC4027G-100UG

Provider

R&D Systems

Product name

Human/Mouse MYF-5 Alexa Fluor® 488-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human MYF-5 in direct ELISAs.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Green dye

Antigen sequence

P13349


Isotype

IgG

Antibody clone number

593128

Vial size

100 ug

Concentration

0.2 mg/ml

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of MYF-5 in C2C12 Mouse Cell Line by Flow Cytometry C2C12 mouse myoblast cell line was stained with Mouse Anti-Human/Mouse MYF-5 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC4027G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules