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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- 702744 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ING2 Recombinant Rabbit Monoclonal Antibody (2H44L53)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody is predicted to react with Monkey, Pig, Cat.
- Antibody clone number
- 2H44L53
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of ASK1 was achieved by transfecting MCF-7 cells with ING2 specific siRNA (Silencer® select Product # s7433 and Product # s7432). Western blot analysis (Fig a) was performed using Whole cell extracts from the MCF-7 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blots were probed with Anti-ING2 Recombinant Rabbit Monoclonal Antibody (Product # 702744, 1:200 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this Western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to ING2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on Whole cell extracts (30 µg lysate) of HCT 116 (Lane 1), Caco-2 (Lane 2), COLO 205 (Lane 3), HT-29 (Lane 4), U-2 OS (Lane 5), MCF-7 (Lane 6) and tissue extracts of Mouse Colon (Lane7). The blots were probed with Anti-ING2 Recombinant Rabbit Monoclonal Antibody (Product # 702744, 1:200 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 32 kDa band corresponding to ING2 was observed across the cell lines and tissues tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Enrichment of endogenous ING2 protein at specific gene loci using Anti-ING2 Recombinant Rabbit Monoclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-ING2 Monoclonal Antibody (Product # 702744, 3 µg) on sheared chromatin from 2 million HeLa cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs for the promoters of the SPP1, TMEM71 positive target gene and SAT2 satellite repeat used as negative target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
