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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- 711779 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ING2 Recombinant Polyclonal Antibody (2HCLC)
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 2HCLC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HCT 116 (Lane 1), Caco-2 (Lane 2), COLO 205 (Lane 3), HT-29 (Lane 4), U-2 OS (Lane 5), HEK-293 (Lane 6) ,Hep G2 (Lane 7) and MCF-7 (Lane 8). The blots were probed with Anti-ING2 Recombinant Rabbit Polyclonal Antibody (Product # 711779, 1:200 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 32 kDa band corresponding to ING2 was observed across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of ING2 was achieved by transfecting MCF7 cells with ING2 specific siRNA (Silencer® select Product # s7433 and Product # s7432). Western blot analysis (Fig a) was performed using whole cell extracts from the MCF-7 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blots were probed with Anti-ING2 Recombinant Rabbit Polyclonal Antibody (Product # 711779, 1:200 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this Western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to ING2.
