MA1-12558

antibody from Invitrogen Antibodies

Targeting: CASP6 MCH2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-12558

Provider

Invitrogen Antibodies

Product name

Caspase 6 Monoclonal Antibody (MCH2 14 1-190)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

A suggested positive control for this product is MCF-7 cell lysate.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

MCH2 14 1-190

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Caspase 6 using a monoclonal antibody (Product # MA1-12558).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Caspase 6 using a monoclonal antibody (Product # MA1-12558).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Caspase 6 Monoclonal Antibody (MCH2 14 1-190) (Product # MA1-12558) and a 72 kDa band corresponding to Caspase 6 was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of Jurkat (Lane 1) and OVAR-3 (Lane 2) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).