Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-77567 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Orexin Receptor 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
Submitted references Orexin A alleviates neuroinflammation via OXR2/CaMKKβ/AMPK signaling pathway after ICH in mice.
Li T, Xu W, Ouyang J, Lu X, Sherchan P, Lenahan C, Irio G, Zhang JH, Zhao J, Zhang Y, Tang J
Journal of neuroinflammation 2020 Jun 15;17(1):187
Journal of neuroinflammation 2020 Jun 15;17(1):187
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) colon cancer cell lysates with Orexin Receptor 2 polyclonal antibody (Product # PA5-77567) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) colon cancer cell lysates with Orexin Receptor 2 polyclonal antibody (Product # PA5-77567) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) colon cancer cell lysates with Orexin Receptor 2 polyclonal antibody (Product # PA5-77567) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) colon cancer cell lysates with Orexin Receptor 2 polyclonal antibody (Product # PA5-77567) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Orexin Receptor 2 in paraformaldehyde-fixed and permeabilized human colon cancer cells. A) Samples were probed with Orexin Receptor 2 polyclonal antibody (Product # PA5-77567) at a dilution of 1:500, and incubated with goat-anti-rabbit-AlexaFluor-555. B) Shows live view of samples. C) Shows nuclei stained image.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Orexin Receptor 2 in rat brain. A) Samples were probed with Orexin Receptor 2 polyclonal antibody (Product # PA5-77567) using a dilution of 1:500, and incubated with Hoechst. B) In the parietal cortex, staining is evident in neural cell bodies. C)In the hippocampus, staining is present in pyramidal cells in the CA1 layer.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Time-course study of OXA, OXR1, and OXR2 using western blot. a Representative protein bands. b Western blot quantitative analyses of OXA, one-way ANOVA, * p < 0.05 vs. sham group. Error bars represent the means +- SD, n = 6 per group. c Western blot quantitative analyses of OXR1, one-way ANOVA, * p < 0.05 vs. sham group. Error bars represent the means +- SD, n = 6 per group. d Western blot quantitative analyses of OXR2, one-way ANOVA, * p < 0.05 vs. sham group. Error bars represent mean +- SD, n = 6 per group