AOR-002-200UL

antibody from Invitrogen Antibodies

Targeting: HCRTR2 OX2R

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AOR-002-200UL

Provider

Invitrogen Antibodies

Product name

Orexin Receptor 2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) colon cancer cell lysates: - 1,2. Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:200).3,4. Anti-Orexin Receptor 2 Antibody , preincubated with Orexin Receptor 2 Blocking Peptide (#BLP-OR002).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:400).3,4. Anti-Orexin Receptor 2 Antibody , preincubated with Orexin Receptor 2 Blocking Peptide (#BLP-OR002).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) colon cancer cell lysates: - 1,2. Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:200).3,4. Anti-Orexin Receptor 2 Antibody , preincubated with Orexin Receptor 2 Blocking Peptide (#BLP-OR002).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:400).3,4. Anti-Orexin Receptor 2 Antibody , preincubated with Orexin Receptor 2 Blocking Peptide (#BLP-OR002).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of OX2R in human colon cancer cells - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized human Colo-205 colon cancer cells using Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:500), followed by goat- Anti-rabbit-AlexaFluor-555 secondary Antibody (red). B.Live view of the same field as in (A). C.Nuclei were visualized with the cell permeable dye Hoechst 33342 (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of OX2R in rat brain - Immunohistochemical staining of rat brain using Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:50), followed by goat Anti-rabbit-AlexaFluor-555 secondary Antibody (1:500), (red). A.In the cerebellum, staining is present in cell bodies of both Golgi type I (Purkinje cells – white arrows) and Golgi type II (green arrows) neurons. Staining is also present in fibers in the molecular layer (ML). B.In the parietal cortex, staining is evident in neural cell bodies. C.In the hippocampus, staining is present in pyramidal cells in the CA1 layer. Note that both cell bodies and prolongations are stained.Hoechst 33342 is used asthe counterstain (blue).