NB100-247

antibody from Novus Biologicals

Targeting: CLSPN

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-247

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-247, RRID:AB_2292037

Product name

Rabbit Polyclonal Claspin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Kinase-independent function of checkpoint kinase 1 (Chk1) in the replication of damaged DNA.

Speroni J, Federico MB, Mansilla SF, Soria G, Gottifredi V
Proceedings of the National Academy of Sciences of the United States of America 2012 May 8;109(19):7344-9

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USP1 deubiquitinase maintains phosphorylated CHK1 by limiting its DDB1-dependent degradation.

Guervilly JH, Renaud E, Takata M, Rosselli F
Human molecular genetics 2011 Jun 1;20(11):2171-81

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ATR/Chk1 pathway is essential for resumption of DNA synthesis and cell survival in UV-irradiated XP variant cells.

Despras E, Daboussi F, Hyrien O, Marheineke K, Kannouche PL
Human molecular genetics 2010 May 1;19(9):1690-701

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NK cells recognize and kill human glioblastoma cells with stem cell-like properties.

Castriconi R, Daga A, Dondero A, Zona G, Poliani PL, Melotti A, Griffero F, Marubbi D, Spaziante R, Bellora F, Moretta L, Moretta A, Corte G, Bottino C
Journal of immunology (Baltimore, Md. : 1950) 2009 Mar 15;182(6):3530-9

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Chk1 and Claspin potentiate PCNA ubiquitination.

Yang XH, Shiotani B, Classon M, Zou L
Genes & development 2008 May 1;22(9):1147-52

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Loss of CHK1 function impedes DNA damage-induced FANCD2 monoubiquitination but normalizes the abnormal G2 arrest in Fanconi anemia.

Guervilly JH, Macé-Aimé G, Rosselli F
Human molecular genetics 2008 Mar 1;17(5):679-89

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Phosphatase type 2A-dependent and -independent pathways for ATR phosphorylation of Chk1.

Li G, Elder RT, Qin K, Park HU, Liang D, Zhao RY
The Journal of biological chemistry 2007 Mar 9;282(10):7287-98

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Human INT6 interacts with MCM7 and regulates its stability during S phase of the cell cycle.

Buchsbaum S, Morris C, Bochard V, Jalinot P
Oncogene 2007 Aug 2;26(35):5132-44

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Regulation of Claspin degradation by the ubiquitin-proteosome pathway during the cell cycle and in response to ATR-dependent checkpoint activation.

Bennett LN, Clarke PR
FEBS letters 2006 Jul 24;580(17):4176-81

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Rad17 phosphorylation is required for claspin recruitment and Chk1 activation in response to replication stress.

Wang X, Zou L, Lu T, Bao S, Hurov KE, Hittelman WN, Elledge SJ, Li L
Molecular cell 2006 Aug 4;23(3):331-41

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Destruction of Claspin by SCFbetaTrCP restrains Chk1 activation and facilitates recovery from genotoxic stress.

Mailand N, Bekker-Jensen S, Bartek J, Lukas J
Molecular cell 2006 Aug 4;23(3):307-18

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Cleavage of claspin by caspase-7 during apoptosis inhibits the Chk1 pathway.

Clarke CA, Bennett LN, Clarke PR
The Journal of biological chemistry 2005 Oct 21;280(42):35337-45

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Claspin Antibody [NB100-247] - Detection of human Claspin on HeLa whole cell lysate using NB100-247.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Claspin Antibody [NB100-247] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody NB100-247 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: Claspin Antibody [NB100-247] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody used for IP at 6 ug/mg lysate. For blotting immunoprecipitated Claspin,was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Claspin Antibody [NB100-247] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Claspin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB