AF6176

antibody from Novus Biologicals

Targeting: BMP5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6176

Provider

Novus Biologicals

Product name

Sheep Polyclonal BMP-5 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse BMP-5 in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human BMP-5 is observed. In direct ELISAs, approximately 100% cross-reactivity with recombinant human BMP-5 is observed, and approximately 4% cross-reactivity with recombinant mouse (rm) BMP-6 and rmBMP-7 is observed, and less than 1% cross-reactivity with rmBMP-8b is observed.

Reactivity

Mouse

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells.

Jovanovic VM, Salti A, Tilleman H, Zega K, Jukic MM, Zou H, Friedel RH, Prakash N, Blaess S, Edenhofer F, Brodski C
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Feb 14;38(7):1662-1676

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse BMP-5 by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse BMP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6176) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BMP-5 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

BMP-5 in Mouse Embryo. BMP-5 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Human/Mouse BMP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6176) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain and muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.