Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF6176 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse BMP-5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse BMP-5 in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human BMP-5 is observed. In direct ELISAs, approximately 100% cross-reactivity with recombinant human BMP-5 is observed, and approximately 4% cross-reactivity with recombinant mouse (rm) BMP-6 and rmBMP-7 is observed, and less than 1% cross-reactivity with rmBMP-8b is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P49003
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells.
Jovanovic VM, Salti A, Tilleman H, Zega K, Jukic MM, Zou H, Friedel RH, Prakash N, Blaess S, Edenhofer F, Brodski C
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Feb 14;38(7):1662-1676
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Feb 14;38(7):1662-1676
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse BMP-5 by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse BMP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6176) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BMP-5 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- BMP-5 in Mouse Embryo. BMP-5 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Human/Mouse BMP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6176) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain and muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.