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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- PA5-97037 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BMP5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AANKRKNQNR NKSSSHQDSS RMSSVGDYNT SEQKQACKKH ELYVSFRDLG WQDWIIAPEG YAAFYCDGEC SFPLNAHMNA TNHAIVQTLV HLMFPDHVPK PCCAPTKLNA ISVLYFDDSS NVILKKYRNM VVRSCGCH; Positive Samples: H460, LO2, SW620, 293T, mouse liver, mouse small intestine, mouse skeletal muscle, rat lung, rat liver, NCI-H460, Mouse lung, Mouse liver, Mouse small intestine
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.67 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Adipose-Derived Mesenchymal Stem Cells-Conditioned Medium Modulates the Expression of Inflammation Induced Bone Morphogenetic Protein-2, -5 and -6 as Well as Compared with Shockwave Therapy on Rat Knee Osteoarthritis.
Cheng JH, Hsu CC, Hsu SL, Chou WY, Wu YN, Kuo CA, Hsu TC, Shiu LY, Jhan SW
Biomedicines 2021 Oct 5;9(10)
Biomedicines 2021 Oct 5;9(10)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of BMP5 in extracts of various cell lines using BMP5 Polyclonal Antibody (Product # PA5-97037) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of BMP5 in paraffin-embedded rat lung using BMP5 Polyclonal Antibody (Product # PA5-97037) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of BMP5 in paraffin-embedded rat brain using BMP5 Polyclonal Antibody (Product # PA5-97037) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of BMP5 in paraffin-embedded human prostate using BMP5 Polyclonal Antibody (Product # PA5-97037) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of BMP5 in paraffin-embedded human esophagus using BMP5 Polyclonal Antibody (Product # PA5-97037) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of BMP5 in paraffin-embedded mouse lung using BMP5 Polyclonal Antibody (Product # PA5-97037) at a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Immunohistochemical analysis for ( A ) BMP2, ( B ) BMP5, and ( C ) BMP6 (x400 magnification), and the percentage of BMP2, BMP5, and BMP6 expression in the articular cartilage of the tibia for each group is shown on the left. The 100 and 200 muL adipose-derived mesenchymal stem cells-conditioned medium groups are indicated as CM1 and CM2, respectively. SW indicates shockwave. * p < 0.05, ** p < 0.01, and *** p < 0.001 as compared with the osteoarthritis (OA) group. Eight rats were used for each group.
