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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF615 - Provider product page
- Provider
- R&D Systems
- Product name
- Human BMP-5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human BMP-5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) BMP-2, rhBMP-4, rhBMP-6, rhBMP-7, and rhBMP-8 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Serum Proteases Potentiate BMP-Induced Cell Cycle Re-entry of Dedifferentiating Muscle Cells during Newt Limb Regeneration.
BMP5 expression in the adult rat brain.
Wagner I, Wang H, Weissert PM, Straube WL, Shevchenko A, Gentzel M, Brito G, Tazaki A, Oliveira C, Sugiura T, Shevchenko A, Simon A, Drechsel DN, Tanaka EM
Developmental cell 2017 Mar 27;40(6):608-617.e6
Developmental cell 2017 Mar 27;40(6):608-617.e6
BMP5 expression in the adult rat brain.
Kusakawa Y, Mikawa S, Sato K
Neuroscience 2015 Jan 22;284:972-87
Neuroscience 2015 Jan 22;284:972-87
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Alkaline Phosphatase Production Induced by BMP-5 and Neutral-ization by Human BMP-5 Antibody. Recombinant Human BMP-5 (Catalog # 615-BMC) induces alkaline phosphatase production in the the MC3T3-E1 mouse preosteoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-5 (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BMP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF615). The ND50 is typically 6-24 µg/mL in the presence of L-ascorbic acid (50 µg/mL).
