Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 11-0428-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD42a Monoclonal Antibody (GR-P), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This GR-P monoclonal antibody reacts with human CD42a, which is also known as glycoprotein IX (GPIX). CD42a is a 22-kDa transmembrane protein that is constitutively expressed on platelets and megakaryocytes. CD42a associates with CD42b, CD42c, and CD42d to form the GPIb-V-IX complex, which binds the von Willebrand factor, an endothelial adhesion protein, to mediate platelet adhesion and activation. This binding results in platelet aggregation at sites of vascular injury. This receptor complex has also been demonstrated to bind thrombin, factors XI and XII, P-selectin, and Mac-1. Finally, a point mutation within the leucine-rich motif of CD42a has been linked to Bernard-Soulier syndrome. Applications Reported: This GR-P antibody has been reported for use in flow cytometric analysis. Applications Tested: This GR-P antibody has been pre-titrated and tested by flow cytometric analysis of human platelets. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- GR-P
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human platelets with Anti-Human CD41 PE (Product # 12-0419-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD42a FITC (right). Total viable cells were used for analysis.
- Conjugate
- Green dye