48-0428-41

antibody from Invitrogen Antibodies

Targeting: GP9 CD42a, GPIX

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

48-0428-41

Provider

Invitrogen Antibodies

Product name

CD42a Monoclonal Antibody (GR-P), eFluor™ 450, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: This GR-P monoclonal antibody reacts with human CD42a, which is also known as glycoprotein IX (GPIX). CD42a is a 22-kDa transmembrane protein that is constitutively expressed on platelets and megakaryocytes. CD42a associates with CD42b, CD42c, and CD42d to form the GPIb-V-IX complex, which binds the von Willebrand factor, an endothelial adhesion protein, to mediate platelet adhesion and activation. This binding results in platelet aggregation at sites of vascular injury. This receptor complex has also been demonstrated to bind thrombin, factors XI and XII, P-selectin, and Mac-1. Finally, a point mutation within the leucine-rich motif of CD42a has been linked to Bernard-Soulier syndrome.

Antibody clone number

GR-P

Concentration

5 µL/Test

References

Submitted references

Turbulence Activates Platelet Biogenesis to Enable Clinical Scale Ex Vivo Production.

Ito Y, Nakamura S, Sugimoto N, Shigemori T, Kato Y, Ohno M, Sakuma S, Ito K, Kumon H, Hirose H, Okamoto H, Nogawa M, Iwasaki M, Kihara S, Fujio K, Matsumoto T, Higashi N, Hashimoto K, Sawaguchi A, Harimoto KI, Nakagawa M, Yamamoto T, Handa M, Watanabe N, Nishi E, Arai F, Nishimura S, Eto K
Cell 2018 Jul 26;174(3):636-648.e18

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Flow analysis of individual blood extracellular vesicles in acute coronary syndrome.

Vagida M, Arakelyan A, Lebedeva A, Grivel JC, Shpektor A, Vasilieva E, Margolis L
Platelets 2017 Mar;28(2):165-173

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Signalling through the platelet glycoprotein Ib-V-IX complex.

Canobbio I, Balduini C, Torti M
Cellular signalling 2004 Dec;16(12):1329-44

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Inducible expression of the megakaryocyte-specific gene glycoprotein IX is mediated through an Ets binding site and involves upstream activation of extracellular signal-regulated kinase.

Eisbacher M, Khachigian LM, Khin TH, Holmes ML, Chong BH
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 2001 Aug;12(8):435-45

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The platelet antigens CD9, CD42 and integrin alpha IIb beta IIIa can be topographically associated and transduce functionally similar signals.

Slupsky JR, Kamiguti AS, Rhodes NP, Cawley JC, Shaw AR, Zuzel M
European journal of biochemistry 1997 Feb 15;244(1):168-75

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Human platelet glycoprotein IX: an adhesive prototype of leucine-rich glycoproteins with flank-center-flank structures.

Hickey MJ, Williams SA, Roth GJ
Proceedings of the National Academy of Sciences of the United States of America 1989 Sep;86(17):6773-7

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of normal human platelets with Anti-Human CD41a PE (Product # 12-0419-42) and Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human CD42a eFluor® 450 (right).