Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
- Other assay [7]
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Validation data
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- Product number
- 38-3800 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSME3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
Submitted references p53 is positively regulated by miR-542-3p.
ARF regulates the stability of p16 protein via REGγ-dependent proteasome degradation.
Regulation of REGγ cellular distribution and function by SUMO modification.
Ubiquitin-independent degradation of cell-cycle inhibitors by the REGgamma proteasome.
Ubiquitin- and ATP-independent proteolytic turnover of p21 by the REGgamma-proteasome pathway.
Aberrant accumulation of PTTG1 induced by a mutated thyroid hormone beta receptor inhibits mitotic progression.
The SRC-3/AIB1 coactivator is degraded in a ubiquitin- and ATP-independent manner by the REGgamma proteasome.
Wang Y, Huang JW, Castella M, Huntsman DG, Taniguchi T
Cancer research 2014 Jun 15;74(12):3218-27
Cancer research 2014 Jun 15;74(12):3218-27
ARF regulates the stability of p16 protein via REGγ-dependent proteasome degradation.
Kobayashi T, Wang J, Al-Ahmadie H, Abate-Shen C
Molecular cancer research : MCR 2013 Aug;11(8):828-33
Molecular cancer research : MCR 2013 Aug;11(8):828-33
Regulation of REGγ cellular distribution and function by SUMO modification.
Wu Y, Wang L, Zhou P, Wang G, Zeng Y, Wang Y, Liu J, Zhang B, Liu S, Luo H, Li X
Cell research 2011 May;21(5):807-16
Cell research 2011 May;21(5):807-16
Ubiquitin-independent degradation of cell-cycle inhibitors by the REGgamma proteasome.
Chen X, Barton LF, Chi Y, Clurman BE, Roberts JM
Molecular cell 2007 Jun 22;26(6):843-52
Molecular cell 2007 Jun 22;26(6):843-52
Ubiquitin- and ATP-independent proteolytic turnover of p21 by the REGgamma-proteasome pathway.
Li X, Amazit L, Long W, Lonard DM, Monaco JJ, O'Malley BW
Molecular cell 2007 Jun 22;26(6):831-42
Molecular cell 2007 Jun 22;26(6):831-42
Aberrant accumulation of PTTG1 induced by a mutated thyroid hormone beta receptor inhibits mitotic progression.
Ying H, Furuya F, Zhao L, Araki O, West BL, Hanover JA, Willingham MC, Cheng SY
The Journal of clinical investigation 2006 Nov;116(11):2972-84
The Journal of clinical investigation 2006 Nov;116(11):2972-84
The SRC-3/AIB1 coactivator is degraded in a ubiquitin- and ATP-independent manner by the REGgamma proteasome.
Li X, Lonard DM, Jung SY, Malovannaya A, Feng Q, Qin J, Tsai SY, Tsai MJ, O'Malley BW
Cell 2006 Jan 27;124(2):381-92
Cell 2006 Jan 27;124(2):381-92
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of (A) MeWo, (B) A375, (C) Jurkat, (D) HeLa, and (E) HL-60 cell lysates using Rb anti-PA28g (N-term) (Product # 38-3800).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts of Caco-2 (Lane 1), HeLa (Lane 2), MCF7 (Lane 3), Neuro-2a (Lane 4), PC-12 (Lane 5), COS-7 (Lane 6), Hep G2 (Lane 7), MOLT4 (Lane 8), Jurkat (Lane 9) and NIH/3T3 (Lane 10). The blots were probed with Anti-PA28-gamma Rabbit Polyclonal Antibody (Product # 38-3800, 2µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate (Product # A27036, 0.4µg/mL, 1:2500 dilution). A ~ 31 kDa band corresponding to PA28-gamma was observed across all cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of PA28-gamma was done on Neuro-2a cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with PA28-gamma Rabbit Polyclonal Antibody (Product # 38-3800, red histogram) or with rabbit isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (Product # A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control..
Supportive validation
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