38-3800

antibody from Invitrogen Antibodies

Targeting: PSME3 Ki, PA28-gamma, PA28G, REG-GAMMA

Immunoprecipitation

Antibody data

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Validation data

PSME3 protein structure - 38-3800 shown in red.

Wang Y, Huang JW, Castella M, Huntsman DG, Taniguchi T
Cancer research 2014 Jun 15;74(12):3218-27

Kobayashi T, Wang J, Al-Ahmadie H, Abate-Shen C
Molecular cancer research : MCR 2013 Aug;11(8):828-33

Wu Y, Wang L, Zhou P, Wang G, Zeng Y, Wang Y, Liu J, Zhang B, Liu S, Luo H, Li X
Cell research 2011 May;21(5):807-16

Chen X, Barton LF, Chi Y, Clurman BE, Roberts JM
Molecular cell 2007 Jun 22;26(6):843-52

Li X, Amazit L, Long W, Lonard DM, Monaco JJ, O'Malley BW
Molecular cell 2007 Jun 22;26(6):831-42

Ying H, Furuya F, Zhao L, Araki O, West BL, Hanover JA, Willingham MC, Cheng SY
The Journal of clinical investigation 2006 Nov;116(11):2972-84

Li X, Lonard DM, Jung SY, Malovannaya A, Feng Q, Qin J, Tsai SY, Tsai MJ, O'Malley BW
Cell 2006 Jan 27;124(2):381-92

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of (A) MeWo, (B) A375, (C) Jurkat, (D) HeLa, and (E) HL-60 cell lysates using Rb anti-PA28g (N-term) (Product # 38-3800).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts of Caco-2 (Lane 1), HeLa (Lane 2), MCF7 (Lane 3), Neuro-2a (Lane 4), PC-12 (Lane 5), COS-7 (Lane 6), Hep G2 (Lane 7), MOLT4 (Lane 8), Jurkat (Lane 9) and NIH/3T3 (Lane 10). The blots were probed with Anti-PA28-gamma Rabbit Polyclonal Antibody (Product # 38-3800, 2µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate (Product # A27036, 0.4µg/mL, 1:2500 dilution). A ~ 31 kDa band corresponding to PA28-gamma was observed across all cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of PA28-gamma was done on Neuro-2a cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with PA28-gamma Rabbit Polyclonal Antibody (Product # 38-3800, red histogram) or with rabbit isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (Product # A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control..