PA5-80952

antibody from Invitrogen Antibodies

Targeting: PSME3 Ki, PA28-gamma, PA28G, REG-GAMMA

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80952

Provider

Invitrogen Antibodies

Product name

PSME3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human PSME3.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PSME3 in Lane A: Jurkat Whole Cell Lysate (µg), Lane B: HepG2 Whole Cell Lysate (30 µg). Samples were probed using a PSME3 Polyclonal Antibody (Product # PA5-80952) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), HRP Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:29 kDa. Observed band size:30 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PSME3 Polyclonal Antibody (Product # PA5-80952) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 30 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of PSME3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with PSME3 Polyclonal Antibody (Product # PA5-80952, 1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human PSME3 in human breast carcinoma with PSME3 Polyclonal Antibody (Product # PA5-80952, 1:10,000 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human PSME3 in human colon carcinoma with PSME3 Polyclonal Antibody (Product # PA5-80952, 1:10,000 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PSME3 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate, Lane B: 0.5 mg A-431 Whole Cell Lysate, Lane C: 0.5 mg MCF7 Whole Cell Lysate, Lane D: 0.5 mg HepG2 Whole Cell Lysate 4 µL with PSME3 Polyclonal Antibody (Product # PA5-80952) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: PSME3 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 34 kDa.