A303-381A

antibody from Invitrogen Antibodies

Targeting: IRF2

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A303-381A

Provider

Invitrogen Antibodies

Product name

IRF2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Localization of IRF2 binding sites by ChIP-Sequencing. Chromatin from mouse bone marrow dendritic cells (BMDCs) was immunoprecipitated with anti-IRF2 A303-381A (lot A303-381A-1) and analyzed by DNA sequencing according to methods available at http://www.weizmann.ac.il/immunology/AmitLab/data-and-method/iChIP/method. The figure illustrates the peak distribution of IRF2 binding within a 156 kb region of chromosome 17 (pink) as detected by anti-IRF2 A303-381A.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Localization of IRF2 binding sites by ChIP-Sequencing. Chromatin from mouse bone marrow dendritic cells (BMDCs) was immunoprecipitated with anti-IRF2 A303-381A (lot A303-381A-1) and analyzed by DNA sequencing according to methods available at http://www.weizmann.ac.il/immunology/AmitLab/data-and-method/iChIP/method. The figure illustrates the peak distribution of IRF2 binding within a 156 kb region of chromosome 17 (pink) as detected by anti-IRF2 A303-381A.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human IRF2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from human erythroleukemia (HEL) cells. Antibodies: Affinity purified rabbit anti-IRF2 antibody A303-381A used for IP at 6 µg/mg lysate. IRF2 was also immunoprecipitated by rabbit anti-IRF2 antibodies A303-379A , A303-380A, and A303-382A, which recognize disparate epitopes. For blotting immunoprecipitated IRF2, A303-380A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.