H00007041-M01

antibody from Abnova Corporation

Targeting: TGFB1I1 ARA55, Hic-5, TSC-5

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007041-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007041-M01, RRID:AB_425715

Product name

TGFB1I1 monoclonal antibody (M01), clone 4B2-D8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant TGFB1I1.

Antigen sequence

MPRSGAPKERPAEPLTPPPSYGHQPQTGSGESSGA
SGDKDHLYSTVCKPRSPKPAAPAAPPFSSSSGVLG
TGLCELDRLLQELNATQFNITDEIMSQFPSSKVAS
GEQKEDQSEDKKRPSLPSSPSPGLPKASATSATLE
LDRLMASLSDFRVQNHLPASGPTQPPVVSSTNEGS
PSPPEPTGKGSLDTMLGLLQSDLSRRGVPTQAKGL
CGSCNKPIAGQVVTALGRAWHPEHFVCGGCSTALG
GSSFFEKDGAPFCPECYFERFSPRCGFCNQPIRHK
MVTALGTHWHPEHFCCVSCGEPFGDEGFHEREGRP
YCRRDFLQLFAPRCQGCQGPILDNYISALSALWHP
DCFVCRECFAPFSGGSFFEHEGRPLCENHFHARRG
SLCATCGLPVTGRCVSALGRRFHPDHFTCTFCLRP
LTKGSFQERAGKPYCQPCFLKLFG


Isotype

IgG

Antibody clone number

4B2-D8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

TGFB1I1 monoclonal antibody (M01), clone 4B2-D8 Western Blot analysis of TGFB1I1 expression in Hela ( Cat # L013V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of TGFB1I1 expression in transfected 293T cell line by TGFB1I1 monoclonal antibody (M01), clone 4B2-D8.Lane 1: TGFB1I1 transfected lysate(47.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged TGFB1I1 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol